| 基于超薄等电聚焦电泳技术的杂交水稻种子纯度鉴定 |
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| 作者姓名： | 严敏 赵延明 赵婷 王晓峰 |
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| 作者单位： | 青岛农业大学植物科技学院，华南农业大学生命科学学院 广州510642，青岛农业大学植物科技学院，新疆维吾尔自治区产品质量监督检验研究所，华南农业大学生命科学学院，广州510642，华南农业大学生命科学学院，青岛266109，青岛266109，乌鲁木齐830009，广州510642 |
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| 基金项目： | 广东省自然科学基金;广东省教育厅自然科学基金 |
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| 摘 要： | 为评价超薄等电聚焦电泳技术鉴定杂交水稻种子纯度的准确性;分别利用超薄等电聚焦电泳技术和田间小区种植技术鉴定杂交水稻64S×G67F1种子纯度,比较两者的吻合程度。结果表明:用超薄等电聚焦电泳技术鉴定的种子纯度和大田种植鉴定的纯度完全吻合;说明超薄等电聚焦电泳技术鉴定杂交水稻种子纯度结果准确可靠。
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| 关 键 词： | 超薄等电聚焦电泳 田间小区种植鉴定 杂交水稻 |
Identification of Genetic purity of Hybrid rice Seeds by Ultrathin-layer Isoelectric Focusing of Seed Proteins |
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| Authors: | Yan Min Zhao Yanming Zhao Ting Wang Xiaofeng |
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| Abstract: | To investigate the accuracy of Ultrathin-layer isoelectric focusing of seed proteins (UTLIEF) in identifying the genetic purity of hybrid rice seeds; UTLIEF and grow-out test were used to test the genetic purity of hybrid rice seeds and the genetic purity determined by UTLIEF was compared with that of grow-out test. Genetic purity determined by UTLIEF was the same as that of grow-out test. Genetic purity of hybrid rice seeds determined by UTLIEF was accurate and reliable. |
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| Keywords: | ultrathin layer isoelectric focusing grow-out test genetic purity hybrid rice seeds |
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