| 富硒酵母的筛选鉴定及其作为鱼饲料的应用研究 |
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| 引用本文： | 凡敏,黄珍,李欣,王志,陈雄,代俊.富硒酵母的筛选鉴定及其作为鱼饲料的应用研究[J].湖北农业科学,2017,56(8). |
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| 作者姓名： | 凡敏 黄珍 李欣 王志 陈雄 代俊 |
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| 作者单位： | 工业发酵湖北省协同创新中心/发酵工程教育部重点实验室/湖北工业大学生物工程与食品学院,武汉,430068 |
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| 基金项目： | 湖北工业大学大学生创新创业训练计划项目 |
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| 摘 要： | 将泡菜汁样品稀释后涂布到含1 g/L Na_2SeO_3的YEPD培养基上,筛选出一株耐硒菌株FXY-4,用不同Na_2SeO_3浓度的YEPD培养基培养,发现Na_2SeO_3的浓度为0.5 g/L时菌株富硒能力最强,为0.54mg/g(干重)。运用分子生物学和生理生化方法鉴定FXY-4为光滑假丝酵母(Candida glabrata)。将FXY-4添加在鱼饲料中喂食锦鲤,探究其对锦鲤的生长与血清酶学指标的影响,结果显示添加菌株FXY-4能提高锦鲤生长、免疫学指标和鱼肉中有机硒的含量。
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| 关 键 词： | 硒 光滑假丝酵母(Candidaglabrata) 鉴定 鱼饲料 |
Isolation,Identification and Application Research of Fish Feed of Selenium-rich Yeast |
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| FAN Min,HUANG Zhen,LI Xin,WANG Zhi,CHEN Xiong,DAI Jun.Isolation,Identification and Application Research of Fish Feed of Selenium-rich Yeast[J].Hubei Agricultural Sciences,2017,56(8). |
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| Authors: | FAN Min HUANG Zhen LI Xin WANG Zhi CHEN Xiong DAI Jun |
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| Abstract: | A selenium-rich strain FXY-4 in pickle juice was screened from YEPD medium containing 1 g/L Na2SeO3. The concentration of Na2SeO3 of strain FXY-4 was 0.54 mg/g (dry weight) in YEPD culture of 0.5 g/L selenium. Using molecular biology and physiological and biochemical methods,FXY-4 was identified as Candida glabrata. Fish feed containing strain FXY-4 was fed to koi. It showed that feed could increase the growth, immunological parameters and organic selenium content of koi. |
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| Keywords: | selenium Candida glabrata identity fish feed |
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