壳聚糖对马蹄金种子萌发及幼苗抗性相关的酶活性的影响

为了提高马蹄金种子的发芽率,增强植株的抗逆性,加速品种推广,改善草坪质量,采用不同浓度壳聚糖溶液处理马蹄金种子并进行萌发和栽培试验。统计了种子的发芽率、幼苗的株高、地上部植株鲜重、根长、根重和根冠比,并对幼苗的苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)四种抗性相关酶的活性进行了测定。结果表明:0.8g/L壳聚糖处理与对照相比发芽率降低,其余浓度的壳聚糖对马蹄金种子萌发和幼苗生长具有明显的促进作用;随着处理浓度的提高,各酶的活性有所升高,0.5g/L达到最佳,但浓度进一步增大,却又表现出一定的抑制作用。试验说明,用适量浓度的壳聚糖溶液对马蹄金浸种,可以提高种子的发芽率,改善苗期的生长状况,提高幼苗的抗逆性,缩短成坪时间。

Effects of the Chitosan on the Sprouting of the Dichondra repens Forst. Seeds and the Activities of Resistance Associated Enzyme from Seedling

In order to increase germination ratio of the seeds,enhance stress-resistance of seedling,accelerate popularization of variety,improve quality of turf,this experiment adopted chitosan of different concentrations to deal with the Dichondra repens Forst.seeds and carried out the sprouting and planting test.The test investigated the germination ratio of seed,plant height,fresh weight of shoot,fresh weight of root,root length and ratio of root to shoot.At the same time,the activities of PAL,SOD,POD and PPO of seedling from different stages(7d,14d,21d) were measured.The results showed that the chitosan of other concentrations promoted evidently the seed germination and seedling growth with the exception of 0.8 g/L in germination,and with the increasing of concentration,the activities of four enzymes all increased.The concentration of 0.5g /L was the best.With the furthering concentration,the inhibitive effect appeared.The experiment indicated that the seeds were soaked in chitosan of feasible concentration,the germination ratio of the seeds,the growing state and the stress-resistance of the seedlings could be improved and the turf establishment time could be shorted.