雷州山羊和隆林山羊遗传多样性的微卫星分析

为了调查中国热带、亚热带主要山羊品种的遗传资源现状,应用14对微卫星引物,检测了隆林山羊和雷州山羊不同分布地区的群体遗传多样性水平。隆林山羊(柳州)、隆林山羊(百棚)、雷州山羊(徐闻)、雷州山羊(海南)各群体平均多态信息含量(PIC)分别为0.645、0.600、0.483、0.518;平均杂合度(H)分别为0.281、0.421、0.121、0.157;平均等位基因数(k)分别为5.17、4.93、4.64、4.5,3组数据综合说明两个山羊品种遗传多样性相对匮乏,群体遗传变异较低;NJ法聚类显示,隆林山羊两个群体聚为一类,再与雷州山羊(徐闻)聚在一起,最后为雷州山羊(海南),这与两个品种的来源及地理分布基本一致。研究结果为中国热带、亚热带山羊品种资源开发利用提供了基础资料和科学数据。

Microsatellite Analysis of Genetic Diversity for Leizhou Goat and Longlin Goat

In order to investigate the genetic resources status of mainly goat breeds in tropical and subtropical region of China, the author detect the genetic diversity of Longlin goats and Leizhou goats which distributed different locations by 14 pairs Microsatellite primers. The result showed that the average polymorphic information content (PIC)of Longlin goat (Liuzhou and Baipeng) and Leizhou goat (Xuwen and Hainan) were 0.645, 0.600, 0.483, 0.518, average heterozygosity were 0.281, 0.421, 0121, 0.157 and average number of alleles were 5.17, 4.93, 4.64, 4.5 respectively, three sets of data indicated that the two goat breeds was lack of genetic diversity relatively and genetic variation was lower; the clustered result of NJ Methods showed that two groups of Longlin goats clustered into one category, then clustered with Leizhou goat (Xuwen) together, for the final was Leizhou goat (Hainan), This was in accordance with the origin and geographical distribution of two breeds, The results of this study provide the basis and scientific data for the goat breeds resources development and utilization in tropical and subtropical region of China.