鸡亚慢性镉中毒后肝脏镉含量的测定与形态学观察

【目的】探讨亚慢性镉中毒鸡肝脏中镉的蓄积情况及显微和超微结构的变化。【方法】将30只50日龄健康海蓝白蛋鸡随机分为3组,对照组饲喂基础日粮,低镉组饲喂基础饲粮 CdCl2140mg/kg,高镉组饲喂基础饲粮 CdCl2210mg/kg,分别在20d、40d、60d取肝脏测定镉含量,并做光镜和电镜观察。【结果】镉在肝中的蓄积量存在时间和剂量效应。加镉组鸡肝细胞发生变性、坏死,细胞内线粒体、内质网等膜性细胞器损害严重。【结论】肝脏是镉重要的靶器官;随着染镉剂量的增加和时间的延长,肝脏的损害越严重。

Investigation on Determination of Cadmium and Observation of Morphology in Liver of Hyline-white Laying Hens with Subchronic Cadmium Toxication

[OBJECTIVE] To study the accumulation of cadmium and the changes of morphology in chicken liver induced by sub chronic cadmium. [METHOD] 30 normal Hyline white laying hens, aged 50 days, were randomly allocated to 3 groups and each group included 10 respectively. In feed, we added 0 mg/kg CdCl2, 140 mg/kg CdCl2, 210 mg/kg CdCl2 separately into control group, low-dosage cadmium group and high-dosage cadmium group. Liver was got to determinate the contents of cadmium and observe changes of microstructure or ultrastructure at 20d?40d and 60d after cadmium exposure. [RESULTS] Accumulation of cadmium in liver showed time-effect curve and dose-effect curve. Denaturation and necrosis of cadmium-group liver cell could be seen, mitochondria and endoplasmic reticulum were enormously damaged. [CONCLUSION] Liver was one of the target organ of cadmium and the damage of liver changed deeper with time goes on and elevation of cadmium dose.