| 斑茅成熟种子愈伤诱导及再生体系的建立 |
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| 引用本文： | 张瑜,鄢家俊,马海天才,白史且,张建波,张劲,彭燕,李达旭.斑茅成熟种子愈伤诱导及再生体系的建立[J].湖北农业科学,2017,56(8). |
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| 作者姓名： | 张瑜 鄢家俊 马海天才 白史且 张建波 张劲 彭燕 李达旭 |
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| 作者单位： | 1. 四川省草原科学研究院,成都,611731;2. 四川四海倍特生态环境工程有限公司,成都,610041;3. 四川农业大学草业科学系,四川 雅安,625014 |
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| 基金项目： | 国家自然科学基金项目，四川省公益性科研院所基本科研业务费项目，四川省植物资源平台建设项目 |
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| 摘 要： | 为建立斑茅Erianthus arundinaceum(Retz.)Jeswiet.]的组织培养再生体系,以斑茅的成熟种子为材料,对愈伤组织诱导、芽分化和根分化中的植物生长调节剂配比及浓度进行了筛选。结果表明,(1)MS中添加0.1~0.2 mg/L的6-BA与1.0~3.0 mg/L的2,4-D愈伤诱导率最高;MS中添加0.5 mg/L的2,4-D时胚性愈伤诱导率达54.29%。(2)MS中添加0.5 mg/L的6-BA与1~2 mg/L的KT更易促进芽的分化,芽分化率71.11%。(3)1/2MS中添加20 g/L的蔗糖促进生根效果最好,根分化率96.97%。
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| 关 键 词： | 斑茅[Erianthusarundinaceum(Retz.)Jeswiet.] 愈伤诱导 再生体系 6-BA 2 4-D KT |
Callus Induction of Mature Seed of Erianthus arundinaceus and Establishment of the Regeneration System |
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| ZHANG Yu,YAN Jia-jun,MAHAI Tian-cai,BAI Shi-qie,ZHANG Jian-bo,ZHANG Jin,PENG Yan,Li Da-xu.Callus Induction of Mature Seed of Erianthus arundinaceus and Establishment of the Regeneration System[J].Hubei Agricultural Sciences,2017,56(8). |
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| Authors: | ZHANG Yu YAN Jia-jun MAHAI Tian-cai BAI Shi-qie ZHANG Jian-bo ZHANG Jin PENG Yan Li Da-xu |
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| Abstract: | For establishing the regeneration system of Erianthus arundinaceus, mature seed of Erianthus arundinaceus was used as test material, the phytohormone combination and phytohormone concentration of callus induction, bud initiation and striking root of Erianthus arundinaceus were screened. The results showed that: ①The highest callus induction efficiency used the MS-medium adding 0.1~0.2 mg/L 6-BA and 1.0~3.0 mg/L 2,4-D. Induction rate of embryonic callus was 54.29% using MS-medium adding 0.5 mg/L 2,4-D. ②Bud initiation was 71.11% using MS-medium adding 0.5 mg/L 6-BA and 1~2 mg/L KT. ③Striking root initiation was 96.97% using 1/2 MS-medium adding 20 g/L Cane sugar. |
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| Keywords: | Erianthus arundinaceum(Retz ) Jeswiet callus induction regeneration system 6-BA 2 4-D KT |
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