副猪嗜血杆菌间接血凝诊断液的制备

【目的】建立一种便于检测副猪嗜血杆菌抗体的方法。【方法】将分离纯化的副猪嗜血杆菌5型超声裂解后,致敏双醛处理的健康绵羊红细胞,制成副猪嗜血杆菌5型间接血凝诊断液,检测该诊断液的活力及特异性。【结果】该诊断液特异性强,仅与副猪嗜血杆菌5型阳性血清发生凝集反应。活力也比较高,达到1:512以上,可以用其进行副猪嗜血杆菌疫苗免疫后抗体检测。【结论】该间接血凝诊断液成功制备为广大兽医化验人员提供一种简单、快速、经济的检测副猪嗜血杆菌抗体的方法,为副猪嗜血杆菌病的诊断和防治提供一种有效的手段

The preparation on Indirect Hemagglutination Diagnostic of Haemophilus parasuis

[OBJECTIVE] A indirect hemagglutination test(IHA)was developed of Haemophilus parasuis. [METHOD] Haemophilus parasuis-5 that had separated and purifitied break with Super a wave ; The indirect hemagglutination diagnostic preparation was obtained by sensitization of sheep red blood cell with the purified and broken Haemophilus parasuis-5 sensitized sheep red cells. The activity and speciality of the diagnostic preparation were tested . [RESULT]the result showed that the speciality of the reverse indirect hemagglutination diagnostic preparation is good. It could only agglutinate the same type positive antibody and could be used to detect Haemophilus parasuis-5 antibody. The activity of the diagnostic preparation is high ,it is 1:512.[CONCLUSION] simple fast economic way to test Haemophilus parasuis antibody with The indirect hemagglutination diagnostic preparation, It is a valid means to diagnose and prevent Haemophilus parasuis