| 免疫方式对Iss抗体产生的影响 |
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| 引用本文： | 樊琛,徐旺烨,曾庆华,王会,郑焕芹. 免疫方式对Iss抗体产生的影响[J]. 湖北农业科学, 2017, 56(7). DOI: 10.14088/j.cnki.issn0439-8114.2017.07.027 |
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| 作者姓名： | 樊琛 徐旺烨 曾庆华 王会 郑焕芹 |
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| 作者单位： | 1. 聊城大学农学院,山东 聊城,252059;2. 东北农业大学动物医学院,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金青年项目 |
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| 摘 要： | 为获得抗大肠杆菌Iss蛋白的抗体,将纯化的GST-Iss蛋白分别免疫雏鸡及小鼠。结果显示,无免疫佐剂、弗氏佐剂免疫雏鸡所产生的Iss抗血清效价仅为1∶100,氢氧化铝佐剂免疫小鼠所产生的Iss抗血清效价为1∶60 000。Iss融合蛋白采用氢氧化铝佐剂免疫小鼠,可获得更强的体液免疫应答,抗体效价更高。
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| 关 键 词： | Iss抗体 免疫佐剂 免疫动物 |
The Influence of Immune Methods on Iss Antibody |
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| FAN Chen,XU Wang-ye,ZENG Qing-hua,WANG Hui,ZHENG Huan-qin. The Influence of Immune Methods on Iss Antibody[J]. Hubei Agricultural Sciences, 2017, 56(7). DOI: 10.14088/j.cnki.issn0439-8114.2017.07.027 |
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| Authors: | FAN Chen XU Wang-ye ZENG Qing-hua WANG Hui ZHENG Huan-qin |
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| Abstract: | In order to obtain the antibody against the Iss protein of E. coli,the purified GST-Iss protein was immunized to chicken and mouse respectively. The results showed that Iss antiserum titer of the immune adjuvant-free,Freund's adjuvant immu-nized chicken was just 1:100,the aluminum hydroxide adjuvant immunized mice generated Iss titer of antiserum to 1:60000. The Iss fusion protein using aluminum hydroxide adjuvant immunized mice can obtain stronger humoral immune responses,an-tibody titer was higher. |
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| Keywords: | Iss antibody immune adjuvant immune animal |
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