邓恩桉继代增殖培养条件研究初报 |
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作者姓名: | 易霭琴 童方平 宋庆安 李贵 丁婕 唐春 |
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作者单位: | 湖南省林业科学院,湖南省林木无性系育种重点实验室,长沙410004,湖南省林业科学院,湖南省林木无性系育种重点实验室,长沙410004,湖南省林业科学院,湖南省林木无性系育种重点实验室,长沙410004,中南林业科技大学,湖南省湘潭县林业局,湖南省林业科学院,湖南省林木无性系育种重点实验室,长沙410004 长沙410004,长沙410004,长沙410004,长沙410004,湘潭411228,长沙410004 |
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摘 要: | 为提高邓恩桉组培苗的增殖系数,降低组培苗生产成本,获得健壮的邓恩桉组培苗,通过不同基本培养基、谷氨酸、培养基不同pH值以及不同光照强度对邓恩桉继代培养增殖的影响试验,找到了邓恩桉的组织培养中继代增殖的适宜条件。结果表明,基本培养基、谷氨酸、pH值和光照强度对试管苗的继代增殖都会产生不同程度的影响。基本培养基以改良H为好,添加20mg/L的谷氨酸有利于试管苗的生长,pH值6.2最为适合,最合适的光照强度为2500lx。因此,适合邓恩桉继代增殖的培养条件为:改良H BA0.2~0.4mg/L NAA0.1~0.2mg/L IBA0.2mg/L LH500mg/L 谷氨酸20mg/L,pH6.2,光照强度2500lx。
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关 键 词: | 邓恩桉 组织培养 继代 增殖系数 培养条件 |
Studies on the Culture Conditions of Eucalyptus dunnii Subculture and Multiplication |
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Authors: | Yi Aiqing Tong Fangping Song Qingan Li Gui Ding Jie Tang Chun |
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Abstract: | In order to improve the multiplication coefficient of Eucalyptus dunnii, reduce the cost of culture and obtain the vigorous test-tube plantlet, culture conditions were optimized through testing the multiplication rate and height of test-tube plantlet under different conditions such as basic media, glutamic acid (Glu), pH, intensity of illumination. Through above research, this culture conditions influenced the subculture and multiplication of test-tube plantlet. Basic medium had effects on subculture, modified H was better than H, MS and modified MS. The culture media with Glu promoted the growth far more effectively than non- Glu ones. pH 6.2 and 2500 lx were the most suitable pH and intensity of illumination. So, the suitable culture conditions of Eucalyptus dunnii were modified H BA 0.2 mg/L NAA 0.1 mg/L IBA 0.2 mg/L LH 500 mg/L Glu20mg/L, pH 6.2, intensity of illumination 2500 lx. |
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Keywords: | Eucalyptus dununii tissue culture subculture multiplication culture conditions |
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