黄芩组培工厂化技术研究 |
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作者姓名: | 李永文 寇凤仙 李 红 周艳珍 温秀荣 |
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作者单位: | 保定职业技术学院农林与生物工程系 河北071051 |
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基金项目: | 河北省科技厅项目“药用植物组培工厂化育苗技术研究”(06225509). |
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摘 要: | 选用黄芩健壮枝条作为材料,进行组培快速繁殖技术研究。结果表明:愈伤组织诱导激素组合以6-BA1.0mg/L NAA0.5mg/L最为有利,所形成愈伤组织生长速度和质量均满足要求;不定芽分化激素组合为6-BA1.0mg/L NAA0.1mg/L,增殖方式为器官发生型再生方式;继代培养采用NAA0.5~0.1mg/L B90.5~0.1mg/L,增殖在10倍以上;生根培养NAA0.5mg/L IBA0.1mg/L,一般15d左右生根,平均根量4~5条,生根率近100%,能显著提高移栽成活率,满足植物组织培养工厂化育苗技术要求。
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关 键 词: | 黄芩 植物组织培养 快速繁殖 |
Study on the Industrialized Rapid Clone Propagation Technology
of Scutellaria baicalensis Georgi |
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Authors: | Li Yongwen Kou Fengxian Li Hong Zhou Yanzhen Wen Xiurong |
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Institution: | Baoding Vocational and Technical College, Hebei 071051 |
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Abstract: | Selects the vigorous and healthy branch of Scutellaria baicalensis Georgi as explants carried on the industrialized rapid clone propagation technology research. The results showed that plant hormone combination of 6-BA1.0mg/L NAA0.5mg/L is good for callus induction, 6-BA1.0mg/L NAA0.1mg/L is suitable for Adventitious buds, NAA0.5~0.1mg/L B90.5~0.1mg/L is good for cultivation of young plant, the coefficient of reproduction is up to 10. NAA0.5mg/L IBA0.1mg/L is best for rootage, rooting rate was 100%, and completely meets the demands of the industrialized rapid clone propagation technology. |
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Keywords: | Scutellaria baicalensis Georgi plant tissue culture rapid clone propagation |
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