阿月浑子组织培养研究初报

阿月浑子是中国西部与华北地区极有发展前景的坚果树种，但其微繁技术至今未有突破，以7年生阿月浑子嫁接树茎段为外植体，进行启动培养试验，对MS、改良MS、Q-L4、DKW 4类培养基，BA、IBA、NAA、GA 4种激素与用量，7%次氯酸钠和0.1%升汞溶液与灭菌时间、葡萄糖和蔗糖与用量、叶酸、D-泛酸钙与LH（水解乳蛋白）3种添加剂与用量的培养效果进行比较分析，确定阿月浑子外植体0.1%升汞溶液灭菌10min 效果最好，最优培养基为1/2DKW+0.5mg/l 6-BA +0.5mg/l IBA + mg/l GA3 +1mg/l叶酸+ 300mg/l LH +1mg/l D-泛酸钙+15g/l葡萄糖+ 6.5g/l琼脂。

Preliminary Studies on Tissue Culture of Pistachio

Pistachio is a potential nut tree in the north and west of China,however,the micropropagation of pistachio is not still successful,this experiment were designed for establishing a tissue culture techniques of pistachio.Shoots collected from grafted tree(7yrs)of pistachio were used as explant in primary culture with different treatments of medium(MS,modified MS,Q-L4,DKW),plant hormone(BA,IBA,NAA,GA),disinfectant(7%NaClO3,0.1%mercuric chloride),addition(folic acid,calcium D-pantothenate and LH),glucose and sucrose,experiment results shown that explant of pistachio were disinfected well with 0.1% mercuric chloride for 10min,the suitable primary medium for pistachio was: 1/2DKW 0.5mg/L 6-BA 0.5mg/L IBA 3.0 mg/L GA3 1mg/L folic acid 300mg/L LH 1mg/L calcium D-pantothenate 15g/L glucose 6.5g/L agar.