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| 处理时间对农杆菌介导甘蓝转化中抗性芽分化率的影响 | |
| 引用本文： | 苑丽霞,王景雪,孙毅,杜建中,李贵全,李永虎.处理时间对农杆菌介导甘蓝转化中抗性芽分化率的影响[J].山东农业科学,2006(2):27-29. |
| 作者姓名： | 苑丽霞 王景雪 孙毅 杜建中 李贵全 李永虎 |
| 作者单位： | 1. 山西农业大学,山西,太谷,030801;山西省农业生物技术研究中心,山西,太原,030031 2. 山西省农业生物技术研究中心,山西,太原,030031 3. 山西农业大学,山西,太谷,030801 |
| 摘 要： | 以甘蓝下胚轴为材料,研究根癌农杆菌转化过程中预培养、感染、共培养各阶段中处理时间对出愈率和抗性芽分化率的影响。结果表明,不同阶段处理时间对甘蓝的出愈率和转化效率有明显的影响,甘蓝转化过程中预培养、感染、共培养的最佳处理时间分别为36h、5min、48h。 |
| 关 键 词： | 甘蓝 根癌农杆菌介导法 处理时间 出愈率 分化率 |
| 文章编号： | 1001-4942（2006）02-0027-03 |
| 收稿时间： | 2005-09-11 |
| 修稿时间： | 2006-02-15 |
Effect of processing time on the transformation efficiency of Brassica oleracea mediated by Agrobacterium tume faciens |
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| YUAN Li-xia,WANG Jing-xue,SUN Yi,DU Jian-zhong,LI Gui-quan,LI Yong-hu.Effect of processing time on the transformation efficiency of Brassica oleracea mediated by Agrobacterium tume faciens[J].Shandong Agricultural Sciences,2006(2):27-29. | |
| Authors: | YUAN Li-xia WANG Jing-xue SUN Yi DU Jian-zhong LI Gui-quan LI Yong-hu |
| Institution: | 1. Shanxi Agricultural University, Taigu 030801, China 2. Agro-biotechnology Research Center of Shanzi Province, Taiyuan 030031, China |
| Abstract: | Effects of three kinds of processing time(pre-cultivation time,infection time and co-cultivation time)on the percentage of culli induced and transformation efficiency mediated by Agrobacterium tumefaciens in Brassica oleracea were studied.The results showed that processing time in different stages had apparent effects on the percentage of culli induced and transformation efficiency.The best three kinds of processing time were 36h,5min and 48h respectively. |
| Keywords: | Brassica oleracea Agrobacterium tumefaciens-mediated Processing time Percentage of culli induced Transformation efficiency |
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