不同发根农杆菌菌株诱导番茄毛状根的研究

采用2×3×3三因子设计,研究番茄种子萌发时间、发根农杆菌菌株和侵染菌液浓度对番茄毛状根诱导率的影响。各种因子的组合均能诱导出毛状根,用PCR技术检测诱导出的毛状根,证实了发根农杆菌Ri质粒的rolB基因已经整合到番茄细胞基因组中。结果表明,番茄种子萌发时间、发根农杆菌菌株和侵染菌液浓度对毛状根诱导率有显著影响,且各因子间存在交互作用。其中番茄子叶萌发8~12 d、侵染菌液浓度为OD6000.4~0.6的发根农杆菌ATCC15834,诱导毛状根发生率最高,可达58.8%,是获得番茄毛状根的适宜组合。

Study on tomato hairy root induction by different

Three factor design of experiment were conducted to investigate the effects of germination time of tomato seeds, Agrobacterium rhizogenes strains and the bacterial cell density on induction rate of tomato hairy roots. All the combination of three factors could induce hairy roots. The results of PCR detection on hairy roots indicated that rolB gene in Ri plasmid of A. rhizogenes was integrated into the genome of tomato cell successfully. The results suggested that all of the three factors influenced the induction rate of hairy roots significantly, and there were interactive effects among different factors. The highest induction rate of hairy root was 58.8%, and it occurred when tomato cotyledon was infected by A. rhizogenes strain ATCC15834 with a bacterial cell density of 0.4-0.6 at OD600 after 8-12 d of germination. This combination might be the optimal condition for the induction of tomato hairy roots.