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| 表达传染性法氏囊vp2基因的重组马立克病疫苗对SPF鸡和商品鸡的免疫保护试验 | |
| 引用本文： | 周雪媚,李永清,佘锐萍,章振华,王宏俊,王德成,熊金茂,彭开松.表达传染性法氏囊vp2基因的重组马立克病疫苗对SPF鸡和商品鸡的免疫保护试验[J].中国预防兽医学报,2007,29(10):791-795. |
| 作者姓名： | 周雪媚 李永清 佘锐萍 章振华 王宏俊 王德成 熊金茂 彭开松 |
| 作者单位： | 1. 中国农业大学,动物医学院,北京,100094;北京市农林科学院,畜牧兽医研究所,北京,100089 2. 北京市农林科学院,畜牧兽医研究所,北京,100089 3. 中国农业大学,动物医学院,北京,100094 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 国内外很多学者都在进行着传染性法氏囊(Infectious bursal disease,IBD)各种基因工程疫苗的研制,IBDV-vp2基因在多种载体中得到表达并取得了较好的保护效力。本实验室已构建了表达IBDV-vp2基因的重组马立克氏病病毒。本研究对该重组马立克氏病病毒疫苗的遗传稳定性以及对SPF鸡和含有母源抗体的商品鸡的免疫保护作用进行了评价。结果表明,103 PFU和104 PFU剂量的重组病毒免疫后对SPF鸡抗传染性法氏囊标准强毒的保护率分别为53%和73%。104 PFU剂量的重组病毒免疫后对含有母源抗体的商品鸡抗传染性法氏囊标准强毒的保护率为87%,结果显示重组疫苗具有一定的应用前景。 |
| 关 键 词： | 传染性法氏囊病病毒 vp2基因 重组马立克氏病病毒 免疫保护 |
| 文章编号： | 1008-0589（2007）10-0791-05 |
| 修稿时间： | 2006-09-19 |
Recombinant MDV expressing vp2 gene of infectious bursal disease virus (IBDV) induced protection against virulent IBDV in chickens with or without maternal antibodies |
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| ZHOU Xue-mei,LI Yong-qing,SHE Rui-ping,ZHANG Zhen-hua,WANG Hong-jun,WANG De-cheng,XIONG Jin-mao,PENG Kai-song.Recombinant MDV expressing vp2 gene of infectious bursal disease virus (IBDV) induced protection against virulent IBDV in chickens with or without maternal antibodies[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(10):791-795. | |
| Authors: | ZHOU Xue-mei LI Yong-qing SHE Rui-ping ZHANG Zhen-hua WANG Hong-jun WANG De-cheng XIONG Jin-mao PENG Kai-song |
| Institution: | 1. Department of Veterinary Medicine, China Agricultural University, Beijing 100094, China; 2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China |
| Abstract: | In the present study,efficacy of the recombinant MDV vaccine was evaluated in SPF and commercial chickens.All chickens were vaccinated with recombinant MDV vaccine at 1 day old and challenged with IBDV CJ801 strain.SPF chickens showed 53 % and 73 % protection when receiving 103 PFU and 104 PFU vaccine doses,respectively.In commercial chickens,the rMDV conferred 87 % protection against virulent IBDV CJ801 strain.These results demonstrated that the recombinant MDV could be a promising vaccine candidate against virulent IBDV. |
| Keywords: | infectious bursal disease virus vp2 gene recombinant MDV protection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |