| 超级稻离体再生培养条件的优化 |
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| 引用本文： | 欧阳琳,洪亚辉,王若仲,周涌. 超级稻离体再生培养条件的优化[J]. 湖南农业大学学报(自然科学版), 2007, 33(5): 522-524 |
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| 作者姓名： | 欧阳琳 洪亚辉 王若仲 周涌 |
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| 作者单位： | 湖南农业大学生命科学技术学院,湖南,长沙,410128;湖南农业大学生命科学技术学院,湖南,长沙,410128;湖南农业大学湖南省植物激素与生长发育重点实验室,湖南,长沙,410128;湖南农业大学湖南省植物激素与生长发育重点实验室,湖南,长沙,410128 |
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| 基金项目： | 国家自然科学基金项目(30600049) |
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| 摘 要： | 以超级稻品种两优培九成熟胚为外植体,研究了外源激素和化学试剂对超级稻离体再生培养的影响.结果表明,成熟胚在MS 2,4-D2mg/L KT1mg/L培养基上,愈伤组织诱导率达94%;愈伤组织在MS 6-BA2mg/L KT1mg/L NAA0.5mg/L培养基上,分化率为76.66%;当分化培养基中添加5mmol/LCuSO4,愈伤组织生长旺盛,分化率提高,可达86.66%.
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| 关 键 词： | 超级稻 组织培养 愈伤组织 |
| 文章编号： | 1007-1032（2007）05-0522-03 |
| 修稿时间： | 2007-05-16 |
Optimization of super rice in vitro cultivation conditions |
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| OUYANG Lin,HONG Ya-hui,WANG Ruo-zhong,ZHOU Yong. Optimization of super rice in vitro cultivation conditions[J]. Journal of Hunan Agricultural University, 2007, 33(5): 522-524 |
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| Authors: | OUYANG Lin HONG Ya-hui WANG Ruo-zhong ZHOU Yong |
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| Affiliation: | a.College of Biological Science and Biotechnology; b.Hunan Provincial Key Laboratory of Phytohorrnones and Growth Development, HNAU, Changsha410128, China |
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| Abstract: | Using mature embryos of super rice variety,Liangyoupei 9,as explants,the effects of exogenous hommones and chemic reagent on the cellar culture of the super rice in vitro were investigated.The results showed that the induction frequency of mature embryos callus on MS 2,4-D 2 mg/L KT 1mg/L medium was 94%;the differentiation frequency of buds on MS 6-BA 2 mg/L KT 1 mg/L NAA 0.5 mg/L medium,used as differentiation medium,was 76.66%;When 5 mmol/L CuSO4 was added to the differentiational medium,it reached as high as 86.66% and the callus grew well. |
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| Keywords: | super rice tissue culture callus |
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