灯盏花病原锈菌扫描电镜制样方法初探*

 应用SEM技术观察灯盏花锈病的病组织（孢子堆）形态，采用两种方法处理样品。结果表明：常规的制样方法适用于该锈菌的SEM观察，孢子的形态保存完好，结构清晰，没有污染，导电性好。只用戊二醛单固定后处理与用戊二醛-锇酸双固定、乙醇梯度脱水、CO₂临界点干燥法，进行SEM制样观察比较，其结果没有显著差异。本文就该病菌SEM样品处理及形态特征进行了初步观察研究，为该病的防治和进一步研究提供和积累基础理论资料。

Preliminary Studies on Preparation Methods of Semsample for Pathogen of Erigeron breviscapus Rust

Erigeron breviscapus is a kind of wild medicinal plant. It is large-scale artificial cultivated in Yunnan province. Erigeron breviscapus is a new host of Puccinia dovrensis. At present, there is only one sample of this pathogen from Xinjiang Tuomuer in China.With the methods of gluteraldehyde-osmiam tetroxide fixation, step alcohol dehydrating, and CO_2 critical point drying to preparation the SEM samples of Puccinia dovrensis. on Erigeron breviscapus in Yunnan, the research by using methods of sample preparation, were scanned of SEM in this paper. The observation results provided a reliable basis for the discrimination and identification of the Puccinia dovrensis.