金柑高效再生体系的建立 |
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引用本文: | 文露清,杨莉,胡威,邓子牛.金柑高效再生体系的建立[J].中国园艺文摘,2011,27(2):10-12. |
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作者姓名: | 文露清 杨莉 胡威 邓子牛 |
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作者单位: | 湖南农业大学园艺园林学院,湖南长沙410128;国家柑橘改良中心长沙分中心,湖南长沙410128 |
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基金项目: | 农业部948项目,现代农业(柑橘)产业技术体系 |
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摘 要: | 以金柑实生苗为试材进行试验,研究不同再生培养基和苗龄及不同暗培养时间对金柑不定茅再生的影响,建立一套高效稳定的金柑组织培养再生体系.结果表明:采用55 d苗龄的无菌苗上胚轴茎段在Ms+6-苄氨基嘌呤(6-BA)2.0 mg/L+а-萘乙酸(NM)1.0mg/L+激动素(KT)1.0 mg/L+3%蔗糖+0.8%琼脂(DH值5.7)再生培养基上,不经过暗培养直接转移至光/暗周期1 6 h/8 h条件下,为诱导金柑不定芽再生的最佳条件,再生率高达90%.
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关 键 词: | 金柑 实生苗 再生体系 |
Establishing Efficient Regeneration System for Genetic Transformation of Fortunella crassifolia |
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Authors: | WEN Lu-qing YANG Li HU Wei DENG Zi-niu |
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Institution: | WEN Lu-qing YANG Li HU Wei DENG Zi-niu |
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Abstract: | Using epicotyls of kumquat (Fortunella crassifolia Swingle) seedlings growing in vitro as explants, an efficient regeneration system was established. The results show that, using 35-day-old epicotyl segments of kumquat, and based on the culture media of MS+6-BA 2.0 mg/L+NAA 1.0 mg/L+KT 1.0 rag/L+ sucrose 3% + agar 0.8% (pH5.7) in the tempreture of 26℃ for a 16/8 h light/ dark cycle and without dark-culture, adventitious shoots regenerated from 90% of the epicotyl explants. |
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Keywords: | Fortunella crassifolia Swingle seedling regeneration system |
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