| 根癌农杆菌Ti和AT质粒基因组中的分泌型信号肽分析 |
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| 引用本文： | 罗灯涛,范继英,范成明,赵明富,何月秋. 根癌农杆菌Ti和AT质粒基因组中的分泌型信号肽分析[J]. 农业生物技术学报, 2006, 14(2): 265-268 |
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| 作者姓名： | 罗灯涛 范继英 范成明 赵明富 何月秋 |
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| 作者单位： | 云南农业大学植物保护学院,昆明,650201 |
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| 摘 要： | 利用SignalP3.0和PrediSi软件结合L值和TMHMM筛选,对根癌农杆菌(Agrobacteriumtumefaciens)C58Cereon菌株的Ti和AT质粒所编码的745个ORF编码蛋白中的信号肽进行了预测,发现35条为分泌型蛋白,其中6条属于RR-motif型信号肽,2条蛋白的信号肽为细菌素-信息素型信号肽,新注释了24条分泌型信号肽,其中1条来自Ti质粒,23条来自AT质粒的蛋白。这些信号肽N结构域的长度变化为2￣19aa,平均为6.8aa,H结构域的长度变化为8￣34aa,平均为17.1aa。
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| 关 键 词： | 信号肽 信号肽预测 根癌农杆菌 质粒 筛选 |
| 文章编号： | 1006-1304（2006）02-0265-04 |
| 收稿时间： | 2005-03-29 |
| 修稿时间： | 2005-06-22 |
Analysis of Sec-type Signal Peptides in the Ti and AT Plasmids of Agrobacterium tumefaciens C58 Cereon |
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| LUO Deng-tao,FAN Ji-ying,FAN Cheng-ming,ZHAO Ming-fu,HE Yue-qiu. Analysis of Sec-type Signal Peptides in the Ti and AT Plasmids of Agrobacterium tumefaciens C58 Cereon[J]. Journal of Agricultural Biotechnology, 2006, 14(2): 265-268 |
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| Authors: | LUO Deng-tao FAN Ji-ying FAN Cheng-ming ZHAO Ming-fu HE Yue-qiu |
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| Affiliation: | Plant Protection College, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | The 745 ORFs of the Ti and AT plasmids of Agrobacterium tumefaciens C58 Cereon were used for signal peptide prediction by comprehensive analyses with Signal P3.0 and PrediSi and screening based on L value and TMHMM. Total 35 sec-type signal peptides including 6 RR-motif and 2 bacteriocins-pheromones signal peptides were found and among them, 24 peptides were annotated newly as signal peptides, 1 for plasmid Ti and 23 for plasmid AT, respectively. Among the 35 signal peptides, the N-domain has 2~19aa with average 6.8aa, and the H-domain had 8~34aa with average 17.1aa. |
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| Keywords: | signal peptide prediction of signal peptide Agrobacterium tumefaciens C58 Cereon plasmid screening |
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