Probiotic microorganisms and antiviral plants reduce mortality and prevalence of WSSV in shrimp (Litopenaeus vannamei) cultured under laboratory conditions |
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| Authors: | Viridiana Peraza-Gó mez,Antonio Luna-Gonzá lez,Á ngel I Campa-Có rdova,Melina Ló pez-Meyer,Jesú s A Fierro-Coronado,& Pí ndaro Á lvarez-Ruiz |
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| Affiliation: | Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Instituto Politécnico Nacional, Unidad Sinaloa, Sinaloa, Mexico;
Centro de Investigaciones Biológicas del Noroeste, Baja California Sur, Mexico |
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| Abstract: | The protective effect of a probiotic mixture (PM) and antiviral plants, against the white spot syndrome virus (WSSV) in Litopenaeus vannamei , was evaluated in three experiments. The PM was composed of four lactic acid bacteria (LAB) and one yeast strain. The plant mixture was composed of Ocimum sanctum and commercial antiviral plants (VPH®, HSV®). Shrimp in each experiment (weighing 2.7±0.7, 11.5±1.3, 11.70±2.5 g) were cultured in 120-L plastic tanks and fed twice a day with commercial feed plus additives (plants or bacteria and yeast). Animals were monitored for the occurrence of WSSV by single-step and nested PCR. The PM and powdered antiviral plants added to the commercial feed showed an increase in survival and a decrease in the prevalence of WSSV in shrimp. The results showed that both the PM and the powdered antiviral plants can provide protection for shrimp against WSSV. |
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| Keywords: | WSSV Litopenaeus vannamei lactic acid bacteria yeast antiviral plants |
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