芥菜型油菜再生体系的影响因素

对新疆芥菜型油菜４个单、双低吕系子叶柄再生频率的影响因素进行了研究,其中子叶柄日龄、６－ＢＡ、ＡｇＮＯ３、卡那霉素浓度及基因型等因素对不定芽的再生率有很大影响。试验结果表明：萌发４～５ｄ的幼苗子叶柄再生频率最高;６－ＢＡ、ＡｇＮＯ４对不定芽分化的促进效果在不同品系中有大差异,通常浓度为４～６ｍｇ／Ｌ。不同品系对卡那霉素的耐受力有很大不同,其中新９５－Ａ在Ｋａｎ１５ｍｇ／Ｌ时仍有４．１７％的绿苗率;

Studies on the Factors Influencing the High Efficient Regeneration of Brassica juncea

In this paper,factors which influenced the high efficient regeneration system of four "single or double low" strains of Xinjiang B. juncea were studied. Among those,the days of cotyledon petiole,concentration of 6-BA,AgNO₃ and Kanamycin,genotype,etc. played important roles. Results indicated:4~5 days of cotyledon petiole had the highest regeneration rate;6-BA and AgNO₃ had different effect among four strains,usually the best concentration was 4~6 mg/L;to these strains,the tolerance to kanamycin was different,strain Xin95-A still had 4.17% green shoot rate when Kanamycin amount to 15 mg/L;root regeneration of those green shoots was easy,and the survival rate was high after transplanted.