Development, characterization and utilization of microsatellite markers in pigeonpea |
| |
Authors: | D A Odeny Jayashree B M Ferguson D Hoisington J Crouch and C Gebhardt |
| |
Institution: | Centre for Development Research (ZEFc), University of Bonn, Walter-Flex Str.3, D-53113 Bonn, Germany, E-mail:;;International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Hyderabad, India;;International Institute of Tropical Agriculture (IITA), PO Box 30709, Nairobi, Kenya;;International Maize and Wheat Improvement Centre (CIMMYT), CP 56130, Mexico;;Max-Planck Institute for Plant Breeding Research (MPIZ), D-50829 Köln, Germany |
| |
Abstract: | Pigeonpea is a major legume of the semi‐arid tropics that has been neglected in terms of molecular breeding. The objectives of this study were to develop microsatellite markers and evaluate their potential for use in pigeonpea genetics and breeding. Two hundred and eight microsatellite loci were isolated by screening a non‐enriched partial genomic library. Primers were designed for 39 microsatellite loci, 20 of which amplified polymerase chain reaction products of the expected size. Nineteen of the primer pairs were polymorphic amongst 15 cultivated and nine wild pigeonpea accessions providing evidence for cross‐species transferability within the genus Cajanus. A total of 98 alleles were detected at the 19 polymorphic loci with an average of 4.9 alleles per locus. The observed heterozygosity ranged from 0.17 to 0.80 with a mean of 0.42 per locus. Less allelic variation (31 alleles) was observed within the cultivated species than across the wild species (92 alleles). The diversity analysis readily distinguished all wild relatives from each other and from the cultivated germplasm. Development of more microsatellites is recommended for future genomic studies in pigeonpea. |
| |
Keywords: | Cajanus cajan microsatellites Simple Sequence Repeat molecular markers |
|
|