十字花科黑腐病菌hrpW基因导入油菜之研究初报

为了研究十字花科植物黑腐病菌（Xanthomonas campestris pathovar campestris，简称Xcc）hrpW基因的功能及Xcc的致病机理，实验以甘蓝型油菜为试验材料，以5~6d无菌苗的带柄子叶为外植体，建立了高效稳定的带柄子叶再生体系，同时探索了影响根癌农杆菌介导转化甘蓝型油菜子叶柄的各种因素；并用农杆菌介导法将hrpW基因导入甘蓝型油菜中，经PCR检测法分析，证明hrpW基因已整合到油菜基因组中。hrpW基因编码HarpinXcc（十字花科植物黑腐病菌Harpin蛋白），该转基因植株的获得，为进一步研究十字花科植物黑腐病菌的致病机理提供了材料，也为选育高效抗病油菜种质奠定了基础。

Preliminary Report on Obtaining Transgenic Brassica napus L. Harboring hrpW Gene of Xanthomonas campestris pv. Campestris

In order to investigate the function of the hrpW gene and the nosogenesis of Xcc, an efficient and stable regeneration system was established in the experiment by using cotyledonary petioles from 5~6 days' aseptical plantlets of Brassica napus as explants. Factors affecting transformation of cotyledonary petioles of Brassica napus mediated by Agrobacterium tumefaciens were explored. The hrpW gene of Xanthomonas campestris pv. campestris was transferred into Brassica napus L. by using the method of Agrobacterium tumefaciens mediating. The hrpW gene having been integrated into the genome of Brassica napus L. was confirmed by PCR analysis. The hrpW gene encodes HarpinXcc (Harpin proteid of Xanthomonas campestris pv. Campestris). The obtaining of transgenic plants could be used to investigate the pathogenesis of Xanthomonas campestris pv. Campestris, and that layed a foundation for selecting the rape idioplasm of efficiently resisting disease.