| 固氮芽孢杆菌GD272的筛选鉴定及其固氮性能研究 |
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| 引用本文： | 张燕春,孙建光,徐晶,胡海燕.固氮芽孢杆菌GD272的筛选鉴定及其固氮性能研究[J].植物营养与肥料学报,2009,15(5):1196-1201. |
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| 作者姓名： | 张燕春 孙建光 徐晶 胡海燕 |
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| 作者单位： | 1.农业部作物营养与施肥重点实验室;中国农业科学院农业资源与农业区划研究所 北京 100081 |
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| 基金项目： | 引进国际先进农业科学技术计划(948计划)，国家高技术研究发展计划(863计划) |
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| 摘 要： | 对所选育出的一株固氮芽孢杆菌（编号为GD272）进行了形态、生理生化测定和接种效果研究。通过16S rDNA基因比对以及生理生化鉴定表明，该菌株属于芽孢杆菌Bacillus sp.。乙炔还原法测定显示该菌株具有较高的固氮酶活性；小白菜盆栽试验看出，接种GD272菌达到了施用化学氮肥的同等效果。菌株GD272在固氮微生物肥料生产中具有较好的开发应用前景。
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| 关 键 词： | 生物固氮 芽孢杆菌 菌种选育 微生物肥料 |
| 收稿时间： | 2008-9-9 |
Isolation and identification and evaluation of nitrogen-fixing bacillus strain GD272 |
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| ZHANG Yan-chun,SUN Jian-guang,XU Jing,HU Hai-yan.Isolation and identification and evaluation of nitrogen-fixing bacillus strain GD272[J].Plant Nutrition and Fertilizer Science,2009,15(5):1196-1201. |
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| Authors: | ZHANG Yan-chun SUN Jian-guang XU Jing HU Hai-yan |
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| Institution: | 1.Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization/ Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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| Abstract: | Global energy shortage and the high cost of chemical fertilizer make the research of biological nitrogen fixation become necessary and urgent. Research on the breeding and biology of nitrogen-fixing bacteria is the base for nitrogen-fixing microbial fertilizer industry. A nitrogen-fixing bacillus strain GD272 was isolated and its morphology, physiology and chemical properties were studied. Identification with 16S rDNA sequencing, morphology, physiology and chemical properties showed that strain GD272 is Bacillus sp. Relative high nitrogenase activity of GD272 was determined with acetylene reduction assay. Green house test indicated that pakchoi inoculated with GD272 grew as well as those supplied with chemical nitrogen fertilizer. Strain GD272 shows a good prospect in nitrogen fixing microbial fertilizer production. |
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| Keywords: | biological nitrogen fixation bacillus strain breeding microbial fertilizer |
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