| 刺葡萄愈伤组织的分化、芽的增殖及生根培养 |
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| 引用本文： | 刘金亮,吕长平,石雪晖,徐艳,陈冲. 刺葡萄愈伤组织的分化、芽的增殖及生根培养[J]. 作物研究, 2005, 19(2): 106-108 |
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| 作者姓名： | 刘金亮 吕长平 石雪晖 徐艳 陈冲 |
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| 作者单位： | 1. 岳麓山风景名胜管理处,湖南,长沙,410012
2. 湖南农业大学园艺园林学院,长沙,410128 |
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| 摘 要： | 以刺葡萄愈伤组织为材料,在B5培养基上附加不同的激素种类及浓度配比,研究了刺葡萄芽的诱导、增殖和生根培养.结果表明,刺葡萄愈伤组织再生苗的分化率很低,仅在B5 0.5 mg/L ZT 0.1 mg/L NAA上有少量分化,分化率为13.3%;增殖培养以B5 BA1.0 mg/L NAA0.05 mg/L为佳,4周可增殖6.4倍;生根培养以B5 0.5 mg/L IBA 0.02mg/L NAA为好,4周平均生根3.4条,根粗达2.4 mm.
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| 关 键 词： | 刺葡萄 组织培养 愈伤组织 分化 增殖 生根 |
| 文章编号： | 1001-5280(2005)02-0106-03 |
| 修稿时间： | 2005-04-10 |
Callus differentiation ,bud multiplication and rooting culture of Vitis davidii Fo(e)x |
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| LIU Jing-liang,LU Chang-ping,SHI Xue-Hui,XU Yan,CHEN Chong. Callus differentiation ,bud multiplication and rooting culture of Vitis davidii Fo(e)x[J]. Crop Research, 2005, 19(2): 106-108 |
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| Authors: | LIU Jing-liang LU Chang-ping SHI Xue-Hui XU Yan CHEN Chong |
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| Abstract: | Calli of Vitis davidii Fox were used as the material and different plant growth regulators were added at various concentrations into B_5 medium to study bud induction,multiplication and rooting culture.The results showed that calli differentiated in a very frequency and only in the medium supplemented with 0.05 mg/L ZT and 0.1 mg/L NAA in which the frequency of differentiation was 13.3%.The optimum medium for multiplication was B_5 supplemented with 1.0 mg/L BA and 0.05 mg/L NAA, in which the plantlets could propagate 6.4 times in 4 weeks.The most suitable medium for rooting was B_5 supplemented with 0.5 mg/L IBA and 0.02 mg/L NAA in which the plantlet could produce 3.4 roots on average with the thickness of the root up to 2.4 mm. |
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| Keywords: | Vitis davidii Fox Callus Differentiation Multiplication Grow root |
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