| 吐温-80对液氮冷冻处理的双歧杆菌的冻伤保护 |
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| 引用本文： | 闫学会,张兰威,冯镇,单艺. 吐温-80对液氮冷冻处理的双歧杆菌的冻伤保护[J]. 乳业科学与技术, 2006, 28(4): 169-171 |
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| 作者姓名： | 闫学会 张兰威 冯镇 单艺 |
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| 作者单位： | 1. 东北农业大学食品学院,哈尔滨,150030
2. 哈尔滨工业大学食品科学与遗传工程研究院 |
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| 摘 要： | 向双歧杆菌培养基中添加吐温-80，可增加双歧杆菌的细胞膜柔韧性和弹性。经液氮冷冻处理24h后，测定双歧杆菌细胞漏出液中乳酸脱氢酶的活力，结果表明添加吐温-80，可显著降低细胞培养液中的乳酸脱氢酶的漏出率（p〈0．05），其中以添加0．025％吐温-80的效果尤为明显。
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| 关 键 词： | 双歧杆菌 吐温-80 乳酸脱氢酶 |
| 文章编号： | 1671-5187（2006）04-0169-03 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-02-21 |
Protection of Tween-80 against Liquid Nitrogen Frozen Damage of Bifidobacterium |
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| Yan Xuehui,Zhang Lanwei,Feng Zhen,Shan Yi. Protection of Tween-80 against Liquid Nitrogen Frozen Damage of Bifidobacterium[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2006, 28(4): 169-171 |
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| Authors: | Yan Xuehui Zhang Lanwei Feng Zhen Shan Yi |
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| Affiliation: | 1 Food College of Northeast Agricultural University, Harbin of Heilongjiang 150030, P. R. China; 2 Food science and genetic engineering, Harbin institute of technology |
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| Abstract: | Adding tween-80 to bifidobacterium culture made the membrane of bifidobacterium more elastic and flexible. After frozen treated by liquid nitrogen for 24h, the result indicated that th6 leak of lactate dehydrogenase in the cell cultured medium decreased significantly, and adding 0.025% tween-80 was more distinct. |
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| Keywords: | bifidobacterium Tween-80 lactate dehydrogenase |
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