窄冠白杨组培快繁与再生体系的建立

 以窄冠白杨为试材，对其组培快繁与再生体系的建立进行了初步的研究。研究表明，抗氧化剂聚乙烯吡咯烷酮（PVP）和抗坏血酸（Vc）能有效降低外植体的褐化；该品种外植体增殖的最适培养基为1/2MS+BA0.3mg/L+NAA0.05mg/L，平均分化率为87%，增殖系数达7.5倍，与其它处理组合均呈极显著差异。硝普钠（SNP）12mg/L极显著地促进叶片不定芽分化，其再生频率与再生芽个数分别达到96.5%与8.2。窄冠白杨适宜的生根培养基为1/2MS+IBA0.3mg/L。

Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidalis in vitro

In this report, tissue culture and regeneration system from leaves in vitro have been extensively investigated utilizing Populus Leucopyramidalis as experiment materials. The results showed that the anti-oxidation materials polyvinylpyrrolidone (PVP) and ascorbic acid (Vc) decreased browning efficiently; the best medium for proliferation was 1/2 MS+ BA0.3mg/L + NAA 0.05mg/L with differentiation percentage of 87% and proliferation multiples of 7.5 respectively, and there was a great significant difference compared with other treatments. The differentiation and regeneration of adventitious shoot from leaves increased significantly in the medium MS containing BA0.3mg/L and NAA0.05mg/L supplemented with 12mg/L sodium nitroprusside (SNP) in which regeneration frequency and average number of shoots were 100% and 12 respectively. The best rooting medium was 1/2 MS+ IBA0.3mg/L .