千瓣莲品种资源的RAPD分析

千瓣莲是中国独特的荷花资源。为阐明其品种资源间遗传关系。利用16条随机引物对来自云南省的5份农家品种和湖北当阳玉泉寺的1份千瓣莲资源进行了RAPD分析。16条引物共扩增出141条谱带,其中多态带81条,占57.45%;6份千瓣莲之间遗传距离较远。千瓣莲在中国具有较丰富的遗传多样性,并存在较大遗传分化;玉泉寺千瓣莲品种与云南省品种之间没有明显遗传分化;云南省内地方品种间存在较大分化,其中姚安千瓣莲最特殊。

Genetic Relationships of Qianban Lotus Cultivars by RAPD Markers

Qianban Lotus, is a special type of lotus in Nelumbo nucifera Gaertn., which stamen and pistil are completely transformed petals and more than one-thousand petals may be observed during all the period of flowering. To discover the genetic relationships among current cultivars, 5 cultivars from three regions in Yunnan province and one from Yuquan Temple, Dangyang, Hubei province were investigated by employing randomly amplified polymorphic DNA (RAPD) markers. The selected sixteen primers amplified 141 bands in total, of which 81 were polymorphic (57.45%). Our result also exhibited that these cultivars had obvious genetic differentiation with relatively distant genetic distance. There is relatively high level of genetic diversity among these cultivars. No significant genetic differentiation was observed between those cultivars from Yunnan and the one from Yuquan Temple. The cultivars from Yunnan province were found to have obvious genetic differentiation, and the Yaoan lotus displayed its unique genetic characteristics at the most.