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苹果叶片蛋白质双向电泳样品制备方法的比较
引用本文:曾广娟,李春敏,张新忠,滕云龙,董文轩. 苹果叶片蛋白质双向电泳样品制备方法的比较[J]. 勤云标准版测试, 2008, 0(8)
作者姓名:曾广娟  李春敏  张新忠  滕云龙  董文轩
作者单位:沈阳农业大学园艺学院,沈阳 110161;河北省农林科学院昌黎果树研究所,昌黎 066600; 中国农业大学园艺植物研究所,北京 100193;
基金项目:河北省自然科学基金资助项目
摘    要: 为探索适用于苹果叶片蛋白质组学研究的样品制备方法,比较了三氯乙酸/丙酮沉淀法(TCA)、酚-甲醇/醋酸铵沉淀法和Tris-HCl提取法等3种总蛋白质的提取方法。制备的样品经双向电泳分离采用银染显色。结果3种方法分别得到450、374和1032个蛋白质点。认为Tris-HCl提取法为最适方法,所得图谱背景清晰,蛋白质信息量最大。

关 键 词:苹果  蛋白质组  双向电泳

Comparison of Protein Extraction Methods for Two-dimensional Electrophoresis in Apple Leaf
Zeng Guangjuan,Li Chunmin,Zhang Xinzhong,Teng Yunlong,Dong Wenxuan. Comparison of Protein Extraction Methods for Two-dimensional Electrophoresis in Apple Leaf[J]. , 2008, 0(8)
Authors:Zeng Guangjuan  Li Chunmin  Zhang Xinzhong  Teng Yunlong  Dong Wenxuan
Affiliation:College of Horticulture, Shenyang Agricultural Univercity, Shenyang 110161;
Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Science, Changli 066600; Institute for Horticultural Plants, China Agricultural University, Beijing
Abstract:In order to develop an efficient protein extraction method suitable for apple proteome analysis, three protocols for total protein extraction in apple leaf, trichloroacetic acid/acetone precipitation (TCA), phenol extraction methanol/ammonium acetate precipitation and Tris-HCl extraction were compared. The extracts were separated by two-dimensional electrophoresis (2-DE) followed by silver staining. The results showed that 450, 374 and 1032 protein spots were detected respectively on 2-DE gels. The Tris-HCl extraction was the most appropriate for apple leaf proteome analysis on account of the highest resolution and more informative 2-DE gels.
Keywords:
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