| SRAP分子标记及其在蔬菜作物上的应用 |
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| 作者姓名： | 赵光伟 徐志红 徐永阳 |
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| 作者单位： | 中国农业科学院郑州果树研究所,郑州 450009 |
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| 基金项目： | "十一五"国家科技支撑计划课题"优质多抗专用西甜瓜育种技术研究及新品种选育" |
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| 摘 要： | SRAP(Sequence-Related Amplified Polymorphism)是一种基于PCR技术的新型分子标记,具有简便、产率中等、稳定、测序方便等优点。它利用独特的引物设计对ORFs（open reading frames）进行扩增,其上游引物长17bp,下游引物长18bp,分别对外显子和内含子进行扩增,因个体不同及内含子、启动子与间隔长度不等而产生多态性。该文在介绍SRAP标记技术和特点的同时,还对其在蔬菜作物遗传图谱构建、遗传多样性分析、比较基因组学、分子标记与基因定位等方面的应用进行了综述。
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| 关 键 词： | SRAP 分子标记 蔬菜 应用 |
SRAP Molecular Marker and its Applications to Vegetable Crops |
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| Authors: | Zhao Guangwei Xu Zhihong Xu Yongyang |
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| Institution: | Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009 |
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| Abstract: | SRAP(Sequence-Related Amplified Polymorphism)is a new molecular marker based on PCR technique,which combines simplicity,reliability,moderate throughput ratio and facile sequencing of selected bands. ORFs was amplified through the special primer set. The forward primers of 17 bp and reverse primers of 18bp amplified extrons and introns respectively.Polymorphism was produced since the varible introns,promoters and spacers among different individuals.The paper summarized SRAP molecular marker and its applications to genetic-map constructions,genetic diversity analysis, comparative genomic research , molecular marker and gene location of vegetable crops. |
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| Keywords: | SRAPzz molecular markerzz vegetablezz applicationzz |
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