Molecular identification of some African strains of Ralstonia solanacearum from eucalypt and potato |
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| Authors: | Joanne Fouché-Weich Stéphane Poussier Danielle Trigalet-Demery Dave Berger Teresa Coutinho |
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| Institution: | (1) Department of Botany, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa;(2) Département des Sciences Biologiques, Unité de Protection des Plantes, Institut National d'Horticulture (INH), Angers, France;(3) Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, 31326 Castanet-Tolosan Cedex, France;(4) Department of Microbiology and Plant Pathology, FABI, University of Pretoria, Pretoria, South Africa |
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| Abstract: | Ralstonia solanacearum is a known bacterial pathogen of eucalypt and potato plants in Africa. A survey was undertaken to detect this pathogen in
eucalypt plantations in South Africa, the Democratic Republic of Congo, and Uganda. Numerous bacterial strains were isolated
from trees with symptoms typical of bacterial wilt, but only seven were positively identified as R. solanacearum. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, based on the hrp (hypersensitive response and pathogenicity) gene region was used to determine and group the biovars of these R. solanacearum strains. The eucalypt isolates and one potato isolate formed a biovar 3 cluster, whereas the two other potato isolates formed
a cluster that corresponded to biovar 2. Amplified fragment length polymorphism (AFLP) analysis confirmed these clusters.
Therefore, PCR-RFLP can be used as a reliable diagnostic technique to enable researchers to rapidly identify the pathogen. |
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| Keywords: | Ralstonia solanacearum Bacterial wilt Diagnostics PCR-RFLP AFLP |
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