| 山薯组培苗瓶内与瓶外生根技术研究 |
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| 引用本文： | 严华兵,方锋,董伟清,卜朝阳,闭志强,李杨瑞.山薯组培苗瓶内与瓶外生根技术研究[J].广西农业科学,2010,41(10):1046-1048. |
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| 作者姓名： | 严华兵 方锋 董伟清 卜朝阳 闭志强 李杨瑞 |
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| 作者单位： | 广西农业科学院生物技术研究所;广西化工研究院;广西农业科学院; |
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| 基金项目： | 广西科技攻关项目，广西农业科学院基本科研业务专项项目，广西大学博士创新基金项目 |
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| 摘 要： | 以山薯组培苗茎段为外植体,进行组培苗瓶内与瓶外生根技术研究。结果表明,山薯组培苗茎段瓶内生根较优培养基配方为1/2MS液体培养基附加0.5 mg/L NAA、0.1 mg/L 6-BA、30.0 g/L蔗糖和1.5 g/L活性炭;在不同月份移栽对生根苗成活率影响较大,其中以4、10月的移栽生根苗成活率显著高于7月;采用瓶外生根方法,山薯组培苗茎段可不经任何生长素处理,经过21 d的培养,即可获得近90%的生根率;瓶外生根苗培养6周后可在基部形成1～3个微型薯,6个月后可形成1.5～4.0 g大小的微型薯。
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| 关 键 词： | 山薯 茎段 瓶内生根 瓶外生根 微型薯 |
In vitro and ex vitro rooting culture of Dioscorea fordii Prain et Burk plantlets |
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| YAN Hua-bing,FANG Feng,DONG Wei-qing,BU Zhao-yang,BI Zhi-qiang,LI Yang-rui.In vitro and ex vitro rooting culture of Dioscorea fordii Prain et Burk plantlets[J].Guangxi Agricultural Sciences,2010,41(10):1046-1048. |
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| Authors: | YAN Hua-bing FANG Feng DONG Wei-qing BU Zhao-yang BI Zhi-qiang LI Yang-rui |
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| Institution: | YAN Hua-bing1,FANG Feng2,DONG Wei-qing1,BU Zhao-yang1,BI Zhi-qiang1,LI Yang-rui3(1 Bio-technology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China,2 Guangxi Research Institute of Chemical Industry,Nanning 530001,3 Guangxi Academy of Agricultural Sciences,China) |
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| Abstract: | The in vitro and ex vitro rooting of D.fordii variety Guihuai No.2 has been carried out using stem of plantlets as explants.The results showed that 1/2MS basic medium supplemented with 0.5 mg/L NAA,0.1 mg/L 6-BA,30.0 g/L sucrose and 1.5 g/L AC in liquid culture was the preferred medium for in vitro rooting.The survival of rooting plantlet was affected by transplanting in different seasons,and found to be higher in April compared to July.Using ex vitro rooting technique,the stem of plantlet were found to hav... |
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| Keywords: | Dioscorea fordii Prain et Burk stem in vitro rooting ex vitro rooting microtuber |
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