乳脂链球菌产胞外多糖的发酵条件

本文通过单因素及正交实验对乳脂链球菌产胞外多糖的发酵条件进行研究, 单因素实验研究了碳源、接种量、起始pH、发酵温度、镁元素和发酵时间对乳脂链球菌胞外多糖产量的影响; 通过正交实验设计研究三个重要因素乳糖添加量、pH和接种量对胞外多糖产量的影响。最佳发酵条件: 乳糖添加量为20g/L, 发酵培养基初始pH5.5, 接种量5.0%, 发酵温度为37℃, 发酵时间24h, 胞外多糖最高产量可达499.81mg/L。

Study on Fermentation Technology of EPS Produced by Streptococcus. cremoris

Fermentation technology of EPS produced by Streptococcu.cremoris was studied through single factor and rthogonal experiment. During single factor test, the influence of different factors on EPS yield was analysized as different carbon sources, different lactose contents, different initial pH, different cultivation temperature, different mineral quantity, and different fermentation time. Orthogonal experiment was carried by combining the three factors, lactose contents, initial pH and inoculums under three different levels. The best fermentation technology was obtained as lactose content 20 g/L, initial cultivation pH5.5, inoculum quantity 5.0％, fermentation temperature 37℃，fermentation time 24h, the most yield being 499.81mg/L.