维生素A、维生素C和维生素E混合物对浙东白鹅繁殖机能的影响

研究了添加VA、VC和VE混合物(1.5 kg/t饲料)对浙东白鹅繁殖性能、血浆激素浓度和下丘脑-垂体-性腺轴基因mRNA水平的影响。结果显示:饲喂维生素混合物可显著提高浙东白鹅第1产蛋周期蛋重和受精率(P<0.05),但对第2产蛋周期产蛋量、蛋重、受精率和孵化率影响有限;饲喂维生素混合物可提高浙东白鹅第2产蛋周期内血浆FSH、P4、HCG和赖抱期、恢复期E2的浓度,降低LH浓度,改变浙东白鹅产蛋周期内FSH波动规律(P<0.05),降低下丘脑内PRLR、垂体内PRL和卵巢内PRLR基因的mRNA水平(P<0.05),提高卵巢内ESR2基因的mRNA水平(P<0.05),对下丘脑内GnRH、垂体内PRL和FSHβ、卵巢内PRL、ESR1和FSHR基因的mRNA水平影响不显著。因此,VA、VC和VE混合物可以通过影响繁殖期内部分血浆激素浓度变化,下调生殖轴PRLR mRNA水平以减弱PRL信号,从而改善浙东白鹅的繁殖性能。

Effects of Vitamin A,C and E Mixture on the Reproductive Function of Zhedong White Goose

This study was conducted to investigate the effects of addition of vitamin A,C and E mixture(1.5 kg/t basal diet) on the reproduction,plasma hormone concentration and mRNA levels of genes in hypothalamus-pituitary-gonadal axis of Zhedong White Goose.The results showed that the mixture significantly increased the egg weight and fertility rate(P<0.05) in the first laying period,but had limited effects on the egg production,egg weight,fertility rate and hatchability rate in the second laying period;and the mixture increased the concentrations of plasma FSH,P4,HCG and E2,and decreased the concentration of LH,and changed FSH pattern(P<0.05) in the second laying period.The mixture also decreased GnRH,PRL and PRLR mRNA levels in the hypothalamus,pituitary and ovary respectively(P<0.05),and increased ESR2 mRNA level in the ovary(P<0.05).However,there was no significant effects on mRNA levels of PRLR in hypothalamus,PRLR,FSHβ in pituitary,ESR1,PRL and FSHR in ovary.Take together,vitamin A,C and E mixture could improve the reproductive performance by affecting some plasma reproductive hormone concentrations and patterns,and reducing PRL signal due to decreasing PRLR mRNA level in Zhedong White Goose.