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麦根腐长蠕孢毒素对小麦过氧化物酶活性和同工酶的影响
引用本文:高必达,吴畏,程晖,杨家书,吴友三. 麦根腐长蠕孢毒素对小麦过氧化物酶活性和同工酶的影响[J]. 湖南农业大学学报(自然科学版), 1992, 0(Z1)
作者姓名:高必达  吴畏  程晖  杨家书  吴友三
作者单位:湖南农学院植物保护系,沈阳农业大学植物保护系,沈阳农业大学植物保护系,沈阳农业大学植物保护系,沈阳农业大学植物保护系 长沙 410128,沈阳 110126,沈阳 110126,沈阳 110126,沈阳 110126
摘    要:用不同浓度的根腐长蠕孢毒素处理小麦5日龄黄化苗、旗叶圆片和线粒体后,抑制了感病品种过氧化物酶(PO)活性,并导致快带区PO同工酶数目减少;促进了抗病品种PO活性,使其同工酶带活性加强.用根腐毒素处理旗叶提取液后,抗病、中抗和感病品种PO活性有不同程度的减弱,抗病性越强,PO受抑程度越小.因此认为,PO是小麦抗根腐病机制的组分之一,可能与线粒体完整性有关,快带区PO同工酶关系尤为密切.

关 键 词:小麦  根腐病  毒素  过氧化物酶  同工酶

EFFECT OF HELMINTHOSPORAL ON THE ACTIVITY AND ISOZYMES OF PEROXIDASE(PO)IN WHEAT
Gao Bida Wu Wei Cheng Hui Yang Jiashu Wu Yousan. EFFECT OF HELMINTHOSPORAL ON THE ACTIVITY AND ISOZYMES OF PEROXIDASE(PO)IN WHEAT[J]. Journal of Hunan Agricultural University, 1992, 0(Z1)
Authors:Gao Bida Wu Wei Cheng Hui Yang Jiashu Wu Yousan
Affiliation:Gao Bida~1 Wu Wei~2 Cheng Hui~2 Yang Jiashu~2 Wu Yousan~2
Abstract:PO activity was inhibited in the susceptible varieties and enhanced in the resistant ones, and isoperoxidases reduced in the former and increased in the latter, after the treatment of etiolated 5 day-old seedlings, flag leaf discs and mitochondria with the toxin helminthosporal of different concentrations.On the other hand, the inhibition to PO varied with the degree of resistance in 3 varieties after the addition of the toxin into the extracts of flag leaves, ie. the more resistant the variety is, the less inhibited the PO activity is. It comes into the conclution that PO might be one of the constituents of root rot resistance in wheat and help the integrity of mitochdrium, especialy the isope-roxidases in fast-migrating zone in PAGE profile.
Keywords:triticum aestivum  root rot  toxin  peroxidase  isoenzyme  
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