Identification and characterization of Pepino mosaic potexvirus in tomato |
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| Authors: | R. A. A. van der Vlugt C. Cuperus J. Vink I. C. M. M. Stijger D.-E. Lesemann J. Th. J. Verhoeven J. W. Roenhorst |
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| Affiliation: | Plant Research International, PO Box 16, NL-6700 AA Wageningen (The Netherlands);e-mail:;Research Station for Floriculture and Glasshouse Vegetables, PO Box 8, NL-2670 AA Naaldwijk (The Netherlands);Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und Biologische Sicherheit, Messeweg 11-12, D-38104, Braunschweig (Germany);Plant Protection Service, PO Box 9102, NL-6700 HC Wageningen (The Netherlands) |
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| Abstract: | At the beginning of 1999, a new virus disease occurred in protected tomato crops in The Netherlands. Initial diagnostic tests revealed the presence of a potexvirus but serological tests ruled out the presence of Potato X potexvirus (PVX). Tests for other potexviruses reported from solanaceous crops provisionally identified the virus as Pepino mosaic potexvirus (PepMV). The virus was purified, and an antiserum was produced, which showed strong reactions with both the type isolate of PepMV from pepino and two other isolates from tomato. Host range and symptomatology of the pepino and tomato isolates of PepMV revealed clear differences from PVX. However, differences were also observed between the pepino and tomato isolates of PepMV. Sequence alignment of DNA fragments of 584 bp derived from the RNA polymerase cistron showed almost 95% identity with the pepino isolate, whereas the identity with PVX appeared to be < 60%. Together, these results identified PepMV as the causal agent of the new virus disease in tomato. Based on the differences from the type isolate from pepino ( Solanum muricatum ), the isolates from tomato should be considered as a distinct strain of PepMV for which the name tomato strain is proposed. |
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