Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate

Summary An electrophoretic procedure was developed to obtain patterns of water soluble proteins from the endosperm halves of seeds to detect the 1B/1R translocation in wheat cultivars. The water soluble proteins were precipitated with Coomassie Brilliant Blue R-250 and separated in a 10% polyacrylamide gel with sodium dodecyl sulfate. This procedure extracted protein bands (Mr 40,000, 44,000 and 45,000) corresponding to the -secalins of the rye parent which were present only in the wheat cultivars carrying 1B/1R translocation. By simplifying extract preparation and providing clear band resolution this procedure facilitates large scale screening of wheat lines for the rye translocation. Proteins with properties of overlapping solubilities are also discussed.Abbreviations CBB - Coomassie Brilliant Blue - DDW - deionized distilled water - DTT - dithiothreitol - HMW - high molecular weight - PAGE - polyacrylamide gel electrophoresis - Mr - relative molecular mass - SDS sodium dodecyl sulfate Contribution 1569 of Agriculture and Agri-Food Canada, Research Centre, Winnipeg, Canada