蝇蛆几丁低聚糖对花生白绢病菌菌丝形态及超微结构的影响

研究了蝇蛆几丁低聚糖对花生白绢病菌菌丝形态及细胞超微结构的影响，结果表明：蝇蛆几丁低聚糖对花生白绢病菌具有很好的抑制作用，质量浓度7．0mg／mL时抑菌效果达到了100％；显微镜下可观察到蝇蛆几丁低聚糖处理引起菌丝顶端膨大，生长扭曲，菌丝内原生质体分布不均匀，部分细胞甚至开始解体；透射电镜观察发现蝇蛆几丁低聚糖处理导致菌丝内原生质体液泡化，出现电子致密物质，原生质膜部分收缩，液泡内有电子致密物质沉积，液泡逐渐融合成大型液泡，细胞受到严重破坏，甚至解体等一系列细胞超微结构的变化。

The Effect of Chitooligosaccharides from Housefly Larvae on theMorphology and Ultrastructure of Corticium rolfsii Saccardo

The effect of Chitooligosaccharides on the growth, morphology and ultrastructure of Corticium rolfsii Saccardo was studied in this paper. Chitooligosaccharides were found to inhibit the radial growth of Corticium rolfsii Saccardo with an optimal effect at concentrations ranging from 1. 0 to 7. 0 mg/mL. Light microscope observations showed that Chitooligosaccharides induce morphological changes, including marked hyphal swelling and distortion. Examination of hyphae of Corticium rolfsii Saccardo by transmission electron microscopy confirmed that fungal cells subjected to Chitooligosaccharides were structurally damaged. Chitooligosaccharides at 1. 5 mg/mL and 3. 0 mg/mL induced cytoplas-mic vacuolation and electron-dense material. Fungal cells were abnormally shaped and markedly damaged. Higher concentrations of Chitooligosaccharides ( 5. 0 mg/mL) cause fungal cells to be seriously damaged and even lysis.