| 葡萄品种资源的SSR鉴定及遗传多样性分析 |
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| 引用本文： | 郝宇,张淑静,张世红,梁海永.葡萄品种资源的SSR鉴定及遗传多样性分析[J].河北农业大学学报,2010,33(1). |
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| 作者姓名： | 郝宇 张淑静 张世红 梁海永 |
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| 作者单位： | 河北农业大学,林学院,河北,保定,071001 |
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| 摘 要： | 用筛选出的12对SSR引物分析了39个葡萄品种的遗传多样性。结果表明:12对引物在上述品种中共扩增出155条谱带,其中多态性带149条,占扩增总带数的96.13%,特异性条带8条,占5%,所扩增的片段大小在50~1 000 bp之间,不同引物扩增的带数在5~24之间,平均12.9条。扩增条带最多的是引物VrZAG67,扩增出24条带;最少的是VrZAG47,仅扩增了5条带。品种间遗传相似系数变异在0.374~0.974之间,在相似系数为0.660的阈值下,可将39份材料分为3大类群。第1类包括"格拉卡"和"美人指"2个品种;第2类包括"玫瑰香"、"红旗特早"、"达米娜"、"红玫瑰"等30个品种;第3类包括"京云"、"奥古斯特"、"无核早红"、"巨峰"等7个品种。
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| 关 键 词： | SSR 葡萄 遗传多样性 聚类分析 |
SSR identification and analysis of genetic diversity of grape cultivars |
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| HAO Yu,ZHANG Shu-jing,ZHANG Shi-hong,LIANG Hai-yong.SSR identification and analysis of genetic diversity of grape cultivars[J].Journal of Agricultural University of Hebei,2010,33(1). |
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| Authors: | HAO Yu ZHANG Shu-jing ZHANG Shi-hong LIANG Hai-yong |
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| Institution: | College of Forestry;Agricultural University of Hebei;Baoding 071001;China |
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| Abstract: | Twelve pairs of SSR primers were used to study genetic diversity among 39 grape varieties.The result showed that 155 bands were amplified and the polymorphic percentage was 96.13%.There are 8 special bands with the percentage of 5%.The number of amplified bands per primer pair ranges from 5 to 24,with an average of 12.9.With the largest number,primer VrZAG67 amplified 24 bands,and the least was 5 by VrZAG47.The genetic similarity coefficient among all grape varieties ranges from 0.374 to 0.974.Cluster analy... |
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| Keywords: | SSR grape genetic diversity cluster analysis |
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