| ITS片段作为轮枝菌DNA条形码的评价研究 |
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| 引用本文： | 段维军,张慧丽,郭立新,顾建锋,陈先锋,张祥林,夏侯阳.ITS片段作为轮枝菌DNA条形码的评价研究[J].植物保护,2013,39(4):72-77. |
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| 作者姓名： | 段维军 张慧丽 郭立新 顾建锋 陈先锋 张祥林 夏侯阳 |
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| 作者单位： | 1. 宁波出入境检验检疫局，宁波315012; 2. 新疆出入境检验检疫局，乌鲁木齐830063;3. 宁波大学，宁波315212 |
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| 摘 要： | 以轮枝菌属中的重要植物病原性轮枝菌及其近似种共13种35个菌株为材料，探讨ITS片段作为轮枝菌DNA条形码的可行性。结果表明，ITS 成功区分了研究涉及的10个种，其 PCR 扩增和测序成功率为97.1%，序列鉴定成功率为94.5%，但对个别亲缘关系较近种的物种鉴别能力较弱。
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| 关 键 词： | 轮枝菌属 种内与种间差异 PCR 扩增与测序成功率 邻接树 |
Evaluation of ITS region for DNA barcoding of Verticillium spp. |
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| Duan Weijun,Zhang Huili,Guo Lixin,Gu Jianfeng,Chen Xianfeng,Zhang Xianglin,Xiahou Yang.Evaluation of ITS region for DNA barcoding of Verticillium spp.[J].Plant Protection,2013,39(4):72-77. |
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| Authors: | Duan Weijun Zhang Huili Guo Lixin Gu Jianfeng Chen Xianfeng Zhang Xianglin Xiahou Yang |
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| Institution: | 1. Ningbo Entry Exit Inspection and Quarantine Bureau, Ningbo315012, China; 2. Xinjiang Entry Exit Inspection and Quarantine Bureau, Urumqi830063, China; 3. Ningbo University, Ningbo315212, China |
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| Abstract: | In this study, the ITS fragment was tested with 35 strains of 13 species of the Verticillium spp. to investigate its feasibility as a DNA barcode. The results revealed that ITS showed appropriate intra and inter specific variations, and could distinguish the investigated 10 species of the genus. It was easy for PCR amplification and sequencing. The correct identification rate is 94.5% for the sequences tested. However, the inter specific differences of ITS in some closely related isolates were more than the intra specific differences. |
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| Keywords: | Verticillium spp intra and inter specific variation PCR and sequencing success rate NJ phylogeny tree |
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