Long-term nitrogen addition further increased carbon sequestration in a boreal forest

In recent decades, global warming and nitrogen (N) deposition have been increasing obviously, which have led to some strong responses in terrestrial ecosystems, especially the carbon (C) cycle. The boreal forest occupies an important position in the global C cycle with its huge C storage. However, the impact of global change such as N deposition on boreal forest ecosystem C cycle has been not very clear. In order to solve this problem, the field experiment of N addition in a boreal forest has been built in the Greater Khingan Mountains of Northeast China since 2011. Four N addition gradients (0, 25, 50, 75 kg N ha^?1 year^?1) were set up to study the response of above- and belowground C pool to N addition. The results showed that the total forest C sequestration of low-, medium- and high-N treatments was 104.4?±?5.9, 20.2?±?2.7 and 5.3?±?0.4 g C/g N, respectively. Aboveground trees were the largest C pool, followed by soil, roots and floor C pool. Low-N increased the input of C by promoting photosynthesis. Trees of Larix gmelini increased the investment in the belowground root system and increased the belowground C pool. High-N reduced the inter-annual litter biomass and decreased litter C:N that accelerated the decomposition of litter, resulting in a reduction in the floor C pool. Low-N increased total soil respiration, while medium- and high-N inhibited heterotrophic respiration and then increased soil C sequestration. The estimation of forest C pool provides valuable data for improving the C dynamic characteristics of boreal forest ecosystem and is of great significance for us to understand the impact of climate change on the global C cycle.