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| 猪饲料中3种大环内酯类药物的高效毛细管电泳(HPCE)检测方法 | |
| 引用本文： | 童敬,饶钦雄,郭平,李海燕,丁双阳.猪饲料中3种大环内酯类药物的高效毛细管电泳(HPCE)检测方法[J].中国农业大学学报,2009,14(1). |
| 作者姓名： | 童敬 饶钦雄 郭平 李海燕 丁双阳 |
| 作者单位： | 1. 中国农业大学,动物医学院/国家兽药残留基准实验室,北京,100193 2. 上海农业科学院质量标准检测所,上海,201106 3. 中华人民共和国江西出入境检验检疫局,南昌,330002 |
| 摘 要： | 建立了猪饲料中螺旋霉素、替米考星和泰乐菌素3种大环内酯类药物的HPCE检测方法.确定了该过程中缓冲溶液的pH、缓冲溶液类型及离子强度等电泳条件,同时对毛细管长度、内径及分离电压等仪器参数进行优化,得到分离的最优条件:在毛细管有效长度50 cm、内径75 μm,分离电压25 kV,40 mmol/L磷酸氢二钠-20mmol/L柠檬酸(pH2.5)缓冲液条件下,3种药物在15 min内得到完全分离.3种药物在10～100 μg/g范围内线性良好,R>0.999 3;饲料中螺旋霉素检测限为3.0 μg/g,替米考星检测限为5.0μg/g,泰乐菌素检测限为8.0 μg/g,添加回收率在74.5%～87.4%,方法的日内变异系数<8.1%,日间变异系数<7.0%.本方法简单快速,分离环境为水相,经济又环保,适用于大量样本的筛选. |
| 关 键 词： | 高效毛细管电泳 螺旋霉素 替米考星 泰乐菌素 饲料检测 |
Determination of macrolides in feeds by capillary electrophoresis |
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| TONG Jing,RAO Qin-xiong,GUO Ping,LI Hai-yan,DING Shuang-yang.Determination of macrolides in feeds by capillary electrophoresis[J].Journal of China Agricultural University,2009,14(1). | |
| Authors: | TONG Jing RAO Qin-xiong GUO Ping LI Hai-yan DING Shuang-yang |
| Institution: | 1.National Reference Laboratories for Veterinary Drug Residue;Department of Veterinary Pharmacology and Toxicology;College of Veterinary Medicine;China Agricultural University;Beijing 100193;2.Institute for Agr-food Standards and Testing;Shanghai Academy of Agricultural Sciences;Shanghai 201106;3.Jiangxi Entry-Exit and Inspection and Quarantine;Nanchang;Jiangxi;330002 |
| Abstract: | A HPCE method has been developed for the separation of three macrolide antibiotics,Spiramycin(SPM),Tilmicosin(TIL) and Tylosin(TYL) in swine feeds.A systematic approach was used to maximise analyte differential electrophoretic mobility by manipulating electrolyte pH,composition and molarity.In addition,some instrumental parameters such as capillary length and diameter and applied voltage were varied.The analysis was performed in a 40 mmol/L Na2HPO4-20 mmol/L Citric acid buffer(pH 2.5) and an applied voltage... |
| Keywords: | HPCE Spiramycin Tilmicosin Tylosin feeds determination |
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