|
|||||
|
|
| 大蒜胚性细胞悬浮系的建立 | |
| 引用本文： | 孟新亚,邵玉春,刘冬霞,黎明,何宁. 大蒜胚性细胞悬浮系的建立[J]. 河南农业科学, 2002, 0(6): 25-27 |
| 作者姓名： | 孟新亚 邵玉春 刘冬霞 黎明 何宁 |
| 作者单位： | 1. 河南农业大学,郑州,450002 2. 郑州市邙金黄河河务局 3. 新郑市植保技术服务站 4. 河南省农业科学院生物技术研究所 |
| 摘 要： | 以中牟 5号大蒜根尖为外植体 ,在 1 1种固体培养基上诱导愈伤组织 ,筛选出最佳培养基MS + 2 ,4-D 0 .5mg/L和MS + 2 ,4-D 2 .0mg/L +KT 0 .5mg/L。愈伤组织形成后在上述1 1种培养基上继代 3～ 4次 ,获得胚性愈伤组织后再转至液体培养基中 ,经强化培养 3个月后获得胚性细胞悬浮系。试验结果表明 :缩短换液时间 (每隔 3～ 4天换液 1次 ) ,可加快胚性细胞悬浮系的建立 |
| 关 键 词： | 大蒜 中牟5号 愈伤组织 胚性细胞悬浮系 |
| 修稿时间： | 2002-02-27 |
Establishment of Embryonic Cell Suspension Culture Line |
|
| MENG Xing ya ,SHAO Yu Chun ,LIU Dong xia ,LI Ming ,HE Ning. Establishment of Embryonic Cell Suspension Culture Line[J]. Journal of Henan Agricultural Sciences, 2002, 0(6): 25-27 | |
| Authors: | MENG Xing ya SHAO Yu Chun LIU Dong xia LI Ming HE Ning |
| Affiliation: | MENG Xing ya 1,SHAO Yu Chun 2,LIU Dong xia 3,LI Ming 1,HE Ning 4 |
| Abstract: | Root tips of the garlic were used as explants in inducement of garlic calli on the ll lcinds of solid media MS+2,4-D 2.0 mg/L+KT 0.5 mg/L (MS 2) and MS+2,4-D 0.5 mg/L (MS 9) was found to be the best media for callus growth.The embryogenesis calli were gained after the formed calli were put on 11 kinds of media for continued culture in 3～4 generations. The embryogenic cell suspension culture line was set up after the calli were transferred to the liquid medium MS-2 and MS-9 for 3 months of intensive clture.The results of the test show that the embryogenic cell suspension culture can be construsted quickly by shortening the period of changing media,e.g changing media every 3 or 4 days. |
| Keywords: | Garlic Zhongmu 5 Calli Embryogenic cell suspension culture line |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |