| 大赖草的组织培养及植株再生的研究 |
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| 引用本文： | 王丽,邹明谦.大赖草的组织培养及植株再生的研究[J].草业科学,1995,12(6):42-44. |
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| 作者姓名： | 王丽 邹明谦 |
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| 作者单位： | 东北师范大学，遗传与细胞研究所 |
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| 摘 要： | 大刺草在成熟胚在含有２ｍｇ／Ｌ２，４－Ｄ的ＭＳ培养上形成颗粒状愈伤组织，减少培养基中的２，４－Ｄ浓度，愈伤组织分化出绿色的芽点。生根培养基为不含任何激素的ＭＳ培养基，有分化能力的愈伤组织可以在保持培养基（ＭＳ＋２ｍｇ／Ｌ２，４－Ｄ＋０．５ｍｇ／ＬＢＡＰ）上长期保存，利用这一培养程序可以快速，大量地繁殖大赖草，文章还对外源激素在植物组织培养中的作用进行了讨论。
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| 关 键 词： | 大赖草 组织培养 繁殖 植株再生 |
TISSUE CULTURE AND PLANT REGENERATION OF ELYMUS GIGANTEUS |
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| Wang Li,Zou Mingqian, Li Yanning,Zhang Genfa.TISSUE CULTURE AND PLANT REGENERATION OF ELYMUS GIGANTEUS[J].Pratacultural Science,1995,12(6):42-44. |
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| Authors: | Wang Li Zou Mingqian Li Yanning Zhang Genfa |
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| Abstract: | Mature embryos of Elymus giganteus formed nodulous calli on the MS medium containing2mg/L2,4-D. The calli started to differentiate green sports of buds after reducing the level of2,4-D in the medium. The medium for inducing roots of plantlets is the free MS medium. Thecalli which have the potentiality of differentiation can be maintained in the medium with 2 mg/L2,4-D and 0.5mg/L BAP for a long period. This cultured system can be used in the fast re-production in vitro of E. giganteus.The role which exagenous hormones play in plant tissue cul-ture was also discussed in this paper. |
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| Keywords: | Elymus giganteus tissue culture rapid propagation |
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