烟粉虱取食感染TYLCV番茄对其解毒酶和保护酶活性的影响

为了明确感染番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的番茄对烟粉虱生理防御的影响,本文采用生化分析法研究了烟粉虱取食感染TYLCV的番茄植株后解毒酶和保护酶活性的变化。结果表明,B型烟粉虱在带毒番茄上取食72h及30d(长期饲养种群)后,其谷胱甘肽S-转移酶(GSTs)和羧酸酯酶(CarE)活性呈现先升高后下降的趋势,而P450酶活性则持续上升,取食30d种群是对照种群的1.99倍;三大保护酶系超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)则在取食72h后,酶活性高于对照,而在感染TYLCV番茄上取食30d后,保护酶活性则持续高于72h及对照处理,分别是对照种群的1.45倍、8.42倍和5.03倍,差异显著;Q型烟粉虱表现趋势与B型烟粉虱相似。研究结果为进一步明确入侵烟粉虱与双生病毒之间的互作、揭示烟粉虱的适应性机制等补充了资料。

Effects of feeding on TYLCV-infected tomato on detoxification enzyme and protective enzymes of Bemisia tabaci (Gennadius)

In order to explore the physiological response of sweetpotato whitefly, Bemisia tabaci (Gennadius) infected with Tomato yellow leaf curl virus (TYLCV), the detoxification enzymes and protective enzymes activity of B. tabaci were measured by using biochemical analysis. The results showed that the activities of GSTs and CarE increased after the B. tabaci biotype B were reared on viruliferous tomato plant for 72 h, and decreased when kept on the viruliferous tomato for 30 d. The P450 activity of B. tabaci biotype B was proved to be increasing significantly by 1.99 fold, compared to B. tabaci reared on the healthy plant. The activities of protective enzymes including SOD, CAT and POD in B. tabaci biotype B increased after feeding on viruliferous plant for 72 h, and continued increasing when reared on the TYLCV infected tomato persistently, reaching 1.45 fold, 8.42 fold, and 5.03 fold with signicant difference compared to the control, respectively. B. tabaci biotype Q exhibited similar trend as biotype B. These results provide more information for demonstration of the mutualism between whitefly and begomovirus and the adaptive mechanism of B. tabaci.