超级稻吉粳88再生体系的建立

以超级稻吉粳88成熟胚作为外植体,通过对基本培养基、激素组合和干燥处理时间的筛选优化试验,以建立超级稻吉粳88的再生体系。结果表明,以NMB作为基本培养基,2,4-D2.0mg/L、NAA0.1mg/L、KT0.2mg/L和蔗糖40g/L组合条件下,能够诱导出质量较好的愈伤组织,诱导率在80%以上;采用滤纸干燥法对愈伤组织进行干燥处理48h,可使愈伤组织分化率和成苗率明显提高,分化率可达95.0%,成苗率可达20.4%。

Establishment of Regeneration System for Mature Embryo of "Jijing88"

In order to establish a regeneration system of "Jijing 88" , we screened the basic media, optimized hor-mone combinationx, screened the best drying time for mature embryoes of Jijing 88. The results showed that using NMB as basic media with 2,4-D 2.0 mg/L, NAA 0.1 mg/L and KT 0.2 mg/L could induce good quality callus and callus induction rates were above 80% . Moreover, after drying of callus for 48 h by filter paper, callus differen-tiation rate and seedling rate were markedly improved, reaching 95.0% and 20.4% , respectively.