平车前多糖抗氧化作用研究 |
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引用本文: | 朱月,李金香. 平车前多糖抗氧化作用研究[J]. 安徽农业科学, 2010, 38(30): 16853-16854,16857. DOI: 10.3969/j.issn.0517-6611.2010.30.055 |
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作者姓名: | 朱月 李金香 |
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作者单位: | 赤峰学院生命科学系,内蒙古赤峰,024000;赤峰学院生命科学系,内蒙古赤峰,024000 |
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摘 要: | [目的]探寻平车前多糖对羟自由基(.OH)和超氧阴离子自由基(O2-.)的清除作用。[方法]在热水浸提、分离纯化获取平车前多糖的基础上,构建"邻二氮菲-Fe2+氧化法产生羟自由基"和"邻苯三酚自氧化反应产生超氧阴离子自由基"的反应体系,通过紫外可见分光光度法研究平车前多糖对体系中产生的羟自由基(.OH)和超氧阴离子自由基(O2-.)的清除作用。[结果]平车前多糖对羟自由基和超氧阴离子自由基均具有一定的清除作用,并在不同浓度范围内对自由基的清除作用呈上升趋势。[结论]平车前多糖具有一定的抗氧化作用。
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关 键 词: | 平车前多糖 羟自由基 超氧阴离子自由基 清除作用 |
Study on Antioxidation of Plantago depressa Polysaccharide |
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Affiliation: | ZHU Yue et al (Department of Life Science,Chifeng College,Chifeng,Inner Mongolia 024000) |
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Abstract: | [Objective]The aim was to explore the scavenging activity of Plantago depressa polysaccharide to hydroxyl free radical(·OH) and superoxide anion free radical(O2-·).[Method]Plantago depressa polysaccharide was extracted by hot water extraction,then isolated and purified.The hydroxyl free radical and superoxide anion free radical was generated by the phenanthroline-Fe2+ system and the autoxidation reaction system of pyrogallol respectively.Spectrophotometry was used to observe the effect of Plantago depressa polysaccharide on the free radical.[Result]The results showed that Plantago depressa polysaccharide had scavenging effects on O2-·and ·OH,and at different concentrations there was a rising trend of the scavenging activity.[Conclusion]It was demonstrated that Plantago depressa polysaccharide had certain antioxidation effect. |
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Keywords: | Plantago depressa polysaccharide Hydroxyl free radical Superoxide anion free radical Scavenging activity |
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