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| Enterocin A在大肠杆菌中的表达及活性检测 | |
| 引用本文： | 赵爱珍,韩文瑜,徐兴然,冯新.Enterocin A在大肠杆菌中的表达及活性检测[J].吉林农业大学学报,2008,30(1):89-92. |
| 作者姓名： | 赵爱珍 韩文瑜 徐兴然 冯新 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062;济南军区总医院动物实验中心,济南,250031 2. 吉林大学畜牧兽医学院,长春,130062 3. 西南大学,重庆,400716 |
| 基金项目： | 国家自然科学基金项目(30340087) |
| 摘 要： | 将含有编码成熟Enterocin A的基因片段BAE2克隆到pET-28 a( )大肠杆菌表达系统中,在IPTG诱导下,工程菌表达了可溶性的融合多肽His tag-enterocin A,以金属鳌和层析对其进行纯化。利用琼脂扩散试验检测表达产物及纯化产物的抑菌活性。结果表明:His tag-enterocin A具有抗李斯特氏菌活性,但对所选用的金黄色葡萄球菌和大肠杆菌不表现抑制作用,显示与Enterocin A相似的抑菌活性。 |
| 关 键 词： | Enterocin A 融合多肽 抑菌活性 |
| 文章编号： | 1000-5684(2008)01-0089-04 |
| 修稿时间： | 2006年8月31日 |
Fusion Expression of Enterocin A in Escherichia coli and Detection of Antibacterial Activity |
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| ZHAO Ai-zhen,HAN Wen-yu,XU Xing-ran,FENG Xin.Fusion Expression of Enterocin A in Escherichia coli and Detection of Antibacterial Activity[J].Journal of Jilin Agricultural University,2008,30(1):89-92. | |
| Authors: | ZHAO Ai-zhen HAN Wen-yu XU Xing-ran FENG Xin |
| Abstract: | The gene fragment BAE2 encoding mature enterocin A peptide was cloned into pET-28 a( )expression system and expressed as soluble fusion peptides in Escherichia coli BL21(DE3) strain by IPTG induction.The recombinant peptide,with His tag at N-terminus,was named as His tag-enterocin A,and was purified and concentrated by use of Immobilized Metal Affinity Chromatography(IMAC).Antibacterial Activity of samples was determined by the agar diffusion assay.The results showed that His tag-enterocin A were active against 3 strains of Listerial ,but ineffective against a Staphylococcus aureus strain and an Escherichia coli strain used,similar to the characteristics of enterocin A. |
| Keywords: | Enterocin A fusion peptide inhibitory activity |
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