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芳樟工厂化育苗技术研究
引用本文:李乾振,吴丽君,陈碧华,王志洁,翁秋媛,洪志猛,沈继南. 芳樟工厂化育苗技术研究[J]. 福建林业科技, 2001, 28(4): 21-24
作者姓名:李乾振  吴丽君  陈碧华  王志洁  翁秋媛  洪志猛  沈继南
作者单位:福建省林业科学研究院,福建,福州,350012
摘    要:通过对芳樟工业原料林良种茎尖的离体培养和繁殖 ,探讨芳樟茎尖诱导、分化、不定芽生长分化、生根的适宜的培养基和适宜的培养条件。结果表明 :适宜芳樟茎尖诱导培养基为MS +6 -BAa~ 2amg .L-1+NAAbmg .L-1,诱导率达 70 %以上 ,适宜分化丛生芽的理想培养基为改良MS +6 -BAa′~ 2a′mg .L-1+NAA0~ 2bmg .L-1+GA0~cmg .L-1芽年增殖系数达4 5 12 以上 ,有效芽苗率达 80 %以上 ,适合生根培养基为 1/2改良MS +NAAb′mg .L-1+IBA0~b′mg .L-1或 1/2改良MS +IBAb′mg .L-1+NAA0~b′mg .L-1,或R +IBAc2 mg .L-1+NAAc1 mg .L-1生根率达 98%以上。移栽土壤基质以黄心土加沙(10∶1)理想 ,移栽成活率可达 85 %以上

关 键 词:芳樟  工业原料林  组织培养  快速繁殖  工厂化育苗
文章编号:1002-7351(2001)04-0021-04

The Technical Research on the Cinnamomum nominale Factory-Styled Breeding
LI Qian zhen,WU Li jun,CHEN Bi hua,WANG Zhi jie,WENG Qiu yuan,HONG Zhi meng,SHEN Ji nan. The Technical Research on the Cinnamomum nominale Factory-Styled Breeding[J]. Journal of Fujian Forestry Science and Technology, 2001, 28(4): 21-24
Authors:LI Qian zhen  WU Li jun  CHEN Bi hua  WANG Zhi jie  WENG Qiu yuan  HONG Zhi meng  SHEN Ji nan
Abstract:The result show that the suitable Cinnamomum nominale stem apex induction culture medium is MS+6-BA a~2a mg·L -1 +NAA b mg·L -1 ,the induction rate reaches 70% and more,the reasonable culture medium suitable for differentiation cluster buds is improved MS+6-BA a′~2a′ mg·L -1 +NAA 0~2b mg·L -1 +GA 0~c mg·L -1 ,the bud annual proliferation coefficient reaches 4.5 12 and more,the effective bud seedling percentage is 80% and more,the culture medium suitable for rooting is 1/2 improved MS+NAA b′ mg·L -1 +IBA 0~b′ mg·L -1 or 1/2 improved MS+IBA b′ mg·L -1 +NAA 0~b′ mg·L -1 or R+IBA c 2 mg·L -1 +NAA c 1 mg·L -1 ,the rooting rate is 98% and more.The reasonable transplantation soil medium substrate is the yellow heart soil with sand (10∶1),the transplantation survival rate may reach 85% and more.
Keywords:Cinnamomum nominale  Industrial raw material forest  Tissue culture  Rapid propagation  Factory styled breeding
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