The antimicrobial spectra of organic compounds with respect to their potential as hay preservatives

Formic acid, sodium diacetate, propionic acid and two of its ammonium salts, ammonium isobutyrate and tributyl phosphate, were examined for their antimicrobial effects with cultures of micro-organisms typical of the microflora of moist hay. The assays, which were conducted at pH 5, 6, 7 and 8, revealed that all potential preservatives discriminated against actinomycetes in particular and were least effective against fungi. In addition, it was apparent that antimicrobial properties were enhanced under slightly acid conditions and for this reason the use of mixtures of organic acids and salts in commercial hay preservatives is justified. Tributyl phosphate not only had the strongest antimicrobial properties of the compounds tested but was also the least affected by pH.