Primary culture of chicken bursal plical epithelium |
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Authors: | T Nunoya M Tajima C Itakura |
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Institution: | Nippon Institute for Biological Science, Tokyo, Japan. |
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Abstract: | Plical epithelial cells were obtained by trypsin-EDTA treatment of chicken bursa of Fabricius and cultured in the presence of type IV collagen. The culture became confluent six to seven days after seeding. The grown cells showed a positive reaction for cytokeratin by immunostaining and had ultrastructural characteristics of the epithelial cells in vivo. The cell culture will be useful for parasitological and virological studies. |
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